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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2010-arch     Display Documents

ID: 546570.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2010-arch
A role for Rho GTPases and cell-cell adhesion in single-cell motility in vivo.
Authors:Kardash, Elena; Reichman-Fried, Michal; Maître, Jean-Léon; Boldajipour, Bijan; Papusheva, Ekaterina; Messerschmidt, Esther-Maria; Heisenberg, Carl-Philipp; Raz, Erez
Date of Publication (YYYY-MM-DD):2010
Title of Journal:Nature Cell Biology
Issue / Number:1
Start Page:47
End Page:53
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Cell migration is central to embryonic development, homeostasis and disease, processes in which cells move as part of a group or individually. Whereas the mechanisms controlling single-cell migration in vitro are relatively well understood, less is known about the mechanisms promoting the motility of individual cells in vivo. In particular, it is not clear how cells that form blebs in their migration use those protrusions to bring about movement in the context of the three-dimensional cellular environment. Here we show that the motility of chemokine-guided germ cells within the zebrafish embryo requires the function of the small Rho GTPases Rac1 and RhoA, as well as E-cadherin-mediated cell-cell adhesion. Using fluorescence resonance energy transfer we demonstrate that Rac1 and RhoA are activated in the cell front. At this location, Rac1 is responsible for the formation of actin-rich structures, and RhoA promotes retrograde actin flow. We propose that these actin-rich structures undergoing retrograde flow are essential for the generation of E-cadherin-mediated traction forces between the germ cells and the surrounding tissue and are therefore crucial for cell motility in vivo.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:nn
Affiliations:MPI für molekulare Zellbiologie und Genetik
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