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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2010-arch     Display Documents



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ID: 546581.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2010-arch
Global analysis of nascent RNA reveals transcriptional pausing in terminal exons.
Authors:Oesterreich, Fernando Carrillo; Preibisch, Stephan W.; Neugebauer, Karla M.
Date of Publication (YYYY-MM-DD):2010
Title of Journal:Molecular Cell
Volume:40
Issue / Number:4
Start Page:571
End Page:581
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Pre-mRNA splicing is catalyzed by the spliceosome, which can assemble on pre-mRNA cotranscriptionally. However, whether splicing generally occurs during transcription has not been addressed. Indeed, splicing catalysis is expected to occur posttranscriptionally in yeast, where the shortness of terminal exons should leave insufficient time for splicing. Here, we isolate endogenous S. cerevisiae nascent RNA and determine gene-specific splicing efficiencies and transcription profiles, using high-density tiling microarrays. Surprisingly, we find that splicing occurs cotranscriptionally for the majority of intron-containing genes. Analysis of transcription profiles reveals Pol II pausing within the terminal exons of these genes. Intronless and inefficiently spliced genes lack this pause. In silico simulations of transcription and splicing kinetics confirm that this pausing event provides sufficient time for splicing before termination. The discovery of terminal exon pausing demonstrates functional coupling of transcription and splicing near gene ends.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:nn
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:4273
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