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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2010-arch     Display Documents



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ID: 546605.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2010-arch
The differential interaction of snRNPs with pre-mRNA reveals splicing kinetics in living cells.
Authors:Huranová, Martina; Ivani, Ivan; Benda, Ales; Poser, Ina; Brody, Yehuda; Hof, Martin; Shav-Tal, Yaron; Neugebauer, Karla M.; Stanĕk, David
Date of Publication (YYYY-MM-DD):2010
Title of Journal:The Journal of Cell Biology
Volume:191
Issue / Number:1
Start Page:75
End Page:86
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Precursor messenger RNA (pre-mRNA) splicing is catalyzed by the spliceosome, a large ribonucleoprotein (RNP) complex composed of five small nuclear RNP particles (snRNPs) and additional proteins. Using live cell imaging of GFP-tagged snRNP components expressed at endogenous levels, we examined how the spliceosome assembles in vivo. A comprehensive analysis of snRNP dynamics in the cell nucleus enabled us to determine snRNP diffusion throughout the nucleoplasm as well as the interaction rates of individual snRNPs with pre-mRNA. Core components of the spliceosome, U2 and U5 snRNPs, associated with pre-mRNA for 15-30 s, indicating that splicing is accomplished within this time period. Additionally, binding of U1 and U4/U6 snRNPs with pre-mRNA occurred within seconds, indicating that the interaction of individual snRNPs with pre-mRNA is distinct. These results are consistent with the predictions of the step-wise model of spliceosome assembly and provide an estimate on the rate of splicing in human cells.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:nn
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:4205
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