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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2010-arch     Display Documents



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ID: 546645.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2010-arch
Interaction between Sec7p and Pik1p: the first clue for the regulation of a coincidence detection signal.
Authors:Gloor, Yvonne; Schöne, Mario; Habermann, Bianca; Ercan, Ebru; Beck, Mike; Weselek, Grit; Müller-Reichert, Thomas; Walch-Solimena, Christiane
Date of Publication (YYYY-MM-DD):2010
Title of Journal:European Journal of Cell Biology
Volume:89
Issue / Number:8
Start Page:575
End Page:583
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Sec7p, a guanine nucleotide exchange factor, regulates the activation of small Arf GTPases, which function in the formation of distinct classes of transport carriers from the Golgi. The recruitment of a subset of Arf effectors depends on the cooperation between these GTPases and phosphatidylinositol 4-phosphate. Here, we show that the catalytic domain of Sec7p interacts with a conserved region of the Golgi phosphatidylinositol 4-kinase Pik1p. We found that Sec7p and Pik1p as well as its product, colocalize at the late Golgi. Gea1p/Gea2p, an alternative pair of Arf activators, do not bind to Pik1p and function on a different Golgi sub-compartment. Sec7p and Pik1p interact with each other and cooperate in the formation of clathrin-coated vesicles. This interaction reveals a distinct role for Sec7p among the Golgi Arf-GEFs and provides a working model for the coordinated generation of Arf-GTP and phosphatiylinositol 4-phosphate as dual signal for specific recruitment of clathrin coats to the late Golgi.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:nn
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:4362
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