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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2010-arch     Display Documents

ID: 546647.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2010-arch
Global analysis reveals SRp20- and SRp75-specific mRNPs in cycling and neural cells.
Authors:Änkö, Minna-Liisa; Morales, Lucia; Henry, Ian; Beyer, Andreas; Neugebauer, Karla M.
Date of Publication (YYYY-MM-DD):2010
Title of Journal:Nature Structural & Molecular Biology
Issue / Number:8
Start Page:962
End Page:970
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Members of the SR protein family of RNA-binding proteins have numerous roles in mRNA metabolism, from transcription to translation. To understand how SR proteins coordinate gene regulation, comprehensive knowledge of endogenous mRNA targets is needed. Here we establish physiological expression of GFP-tagged SR proteins from stable transgenes. Using the GFP tag for immunopurification of mRNPs, mRNA targets of SRp20 and SRp75 were identified in cycling and neurally induced P19 cells. Genome-wide analysis showed that SRp20 and SRp75 associate with hundreds of distinct, functionally related groups of transcripts that change in response to neural differentiation. Knockdown of either SRp20 or SRp75 led to up- or downregulation of specific transcripts, including identified targets, and rescue by the GFP-tagged SR proteins proved their functionality. Thus, SR proteins contribute to the execution of gene-expression programs through their association with distinct endogenous mRNAs.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:nn
Affiliations:MPI für molekulare Zellbiologie und Genetik
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