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          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents



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ID: 548593.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
A transcriptional roadmap to the induction of pluripotency in somatic cells
Authors:Wang, Y.; Mah, N.; Prigione, A.; Wolfrum, K.; Andrade-Navarro, M. A.; Adjaye, J.
Language:English
Date of Publication (YYYY-MM-DD):2010-03-24
Copyright:© 2010 Springer, Part of Springer Science+Business Media
Audience:Experts Only
Abstract / Description:Human embryonic stem (ES) cells possess an enormous potential for applications in regenerative medicine. However, these cells have several inevitable hurdles limiting their clinical applications, such as transplant rejection and embryo destruction. A milestone recently achieved was the derivation of induced pluripotent stem (iPS) cells by over-expressing combinations of defined transcription factors, namely, OCT4, SOX2, NANOG, and LIN28 or OCT4, SOX2, KLF4, and c-MYC. Human iPS cells exhibit many characteristics identical to those of inner cell mass-derived ES cells. Here, we summarize the generation of human fibroblast-derived iPS cells and discuss the promises and limitations of their use. In addition, by utilising numerous published transcriptome datasets related to ES cells, fibroblast-derived iPS cells, partially induced pluripotent stem cells (PiPSC) and wild type fibroblasts, we reveal similarities (self-renewal signature) and differences (donor cell-type and PiPSC signatures) in genes and associated signaling pathways operative in the induction of pluripotency in fibroblasts. In particular, we highlight that induction of ground state pluripotency is also favoured by the inhibition of epithelial mesenchymal transition (EMT) and hence the induction of mesenchymal epithelial transition (MET). We anticipate that these findings might aid in the establishment of more efficient protocols for inducing pluripotency in somatic cells.
Free Keywords:Cellular reprogramming
EMT
MET
Gene regulatory networks
iPS cells
ES cells
PiPSC
Self-renewal
Pluripotency
Comment of the Author/Creator:Email: adjaye@molgen.mpg.de
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:1.Max Delbrück Center for Molecular Medicine Computational Biology and Data Mining Group Robert-Rössle-Str. 10 Berlin 13125 Germany.
Identifiers:ISSN:1550-8943 [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=... [ID No:2]
DOI:10.1007/s12015-010-9137-2 [ID No:3]
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