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          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents



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ID: 552361.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
An ES-like pluripotent state in FGF-dependent murine iPS cells
Authors:Di Stefano, B.; Buecker, C.; Ungaro, F.; Prigione, A.; Chen, H. H.; Welling, M.; Eijpe, M.; Mostoslavsky, G.; Tesar, P.; Adjaye, J.; Geijsen, N.; Broccoli, V.
Language:English
Date of Publication (YYYY-MM-DD):2010
Title of Journal:PLoS ONE
Volume:5
Issue / Number:12
Start Page:e16092
End Page:e16092
Copyright:© 2010 Di Stefano et al
Review Status:not specified
Audience:Experts Only
Abstract / Description:Recent data demonstrates that stem cells can exist in two morphologically, molecularly and functionally distinct pluripotent states; a naive LIF-dependent pluripotent state which is represented by murine embryonic stem cells (mESCs) and an FGF-dependent primed pluripotent state represented by murine and rat epiblast stem cells (EpiSCs). We find that derivation of induced pluripotent stem cells (iPSCs) under EpiSC culture conditions yields FGF-dependent iPSCs from hereon called FGF-iPSCs) which, unexpectedly, display naive ES-like/ICM properties. FGF-iPSCs display X-chromosome activation, multi-lineage differentiation, teratoma competence and chimera contribution in vivo. Our findings suggest that in 129 and Bl6 mouse strains, iPSCs can dominantly adopt a naive pluripotent state regardless of culture growth factor conditions. Characterization of the key molecular signalling pathways revealed FGF-iPSCs to depend on the Activin/Nodal and FGF pathways, while signalling through the JAK-STAT pathway is not required for FGF-iPS cell maintenance. Our findings suggest that in 129 and Bl6 mouse strains, iPSCs can dominantly adopt a naive pluripotent state regardless of culture growth factor conditions.
Comment of the Author/Creator:E-mail: ngeijsen@mgh.harvard.edu (NG); broccoli.vania@hsr.it (VB)
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:Stem Cells and Neurogenesis Unit, Division of Neuroscience, San Raffaele Scientific Institute, Milan, Italy
Center for Regenerative Medicine, Harvard Medical School, Massachusetts General Hospital, Boston, Massachusetts, United States of America
Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts, United States of America
Department of Cell Biology, Erasmus Medical Centre, Rotterdam, The Netherlands
Department of Stem Cell Biology and Regenerative Medicine, Boston University School of Medicine, Boston, Massachusetts, United States of America
Department of Genetics, Case Western Reserve University, Cleveland, Ohio, United States of America
Hubrecht Institute for Developmental Biology and Stem Cell Research, Utrecht, The Netherlands
Fondazione IRCCS Policlinico San Matteo, Pavia, Italy
Department of Clinical Sciences of Companion Animals, Utrecht University Veterinary School, Utrecht, The Netherlands
Identifiers:ISSN:1932-6203 (Electronic) [ID No:1]
DOI:10.1371/journal.pone.0016092 [ID No:2]
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