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          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents

ID: 552731.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
Global transcriptomic analysis of murine embryonic stem cell-derived brachyury (T) cells
Authors:Doss, M. X.; Wagh, V.; Schulz, H.; Kull, M.; Kolde, R.; Pfannkuche, K.; Nolden, T.; Himmelbauer, H.; Vilo, J.; Hescheler, J.; Sachinidis, A.
Date of Publication (YYYY-MM-DD):2010-03
Title of Journal:Genes to Cells
Issue / Number:3
Start Page:209
End Page:228
Copyright:© 2010 John Wiley & Sons, Inc.
Review Status:not specified
Audience:Experts Only
Abstract / Description:Brachyury(+) mesodermal cell population with purity over 79% was obtained from differentiating brachyury embryonic stem cells (ESC) generated with brachyury promoter driven enhanced green fluorescent protein and puromycin-N-acetyltransferase. A comprehensive transcriptomic analysis of brachyury(+) cells enriched with puromycin application from 6-day-old embryoid bodies (EBs), 6-day-old control EBs and undifferentiated ESCs led to identification of 1573 uniquely up-regulated and 1549 uniquely down-regulated transcripts in brachyury(+) cells. Furthermore, transcripts up-regulated in brachyury(+) cells have overrepresented the Gene Ontology annotations (cell differentiation, blood vessel morphogenesis, striated muscle development, placenta development and cell motility) and Kyoto Encyclopedia of Genes and Genomes pathway annotations (mitogen-activated protein kinase signaling and transforming growth factor beta signaling). Transcripts representing Larp2 and Ankrd34b are notably up-regulated in brachyury(+) cells. Knockdown of Larp2 resulted in a significantly down-regulation BMP-2 expression, and knockdown of Ankrd34b resulted in alteration of NF-H, PPARgamma and PECAM1 expression. The elucidation of transcriptomic signatures of ESCs-derived brachyury(+) cells will contribute toward defining the genetic and cellular identities of presumptive mesodermal cells. Furthermore, there is a possible involvement of Larp2 in the regulation of the late mesodermal marker BMP-2. Ankrd34b might be a positive regulator of neurogenesis and a negative regulator of adipogenesis.
Comment of the Author/Creator:E-mail:
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:Center of Physiology and Pathophysiology, Institute of Neurophysiology, and Center of Molecular Medicine, University of Cologne (CMMC), Robert-Koch Str. 39, 50931 Cologne, Germany
Max-Delbrueck-Center for Molecular Medicine – MDC, Robert-Rössle Str. 10, 13092 Berlin, Germany
Institute of Computer Science, University of Tartu, Liivi 2, 50409 Tartu, Estonia and Quretec Ltd, Ulikooli 6a, Tartu, Estonia
Identifiers:ISSN:1365-2443 (Electronic) [ID No:1]
DOI:10.1111/j.1365-2443.2010.01390.x [ID No:2]
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