Please note that eDoc will be permanently shut down in the first quarter of 2021!      Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents



  history
ID: 552731.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
Global transcriptomic analysis of murine embryonic stem cell-derived brachyury (T) cells
Authors:Doss, M. X.; Wagh, V.; Schulz, H.; Kull, M.; Kolde, R.; Pfannkuche, K.; Nolden, T.; Himmelbauer, H.; Vilo, J.; Hescheler, J.; Sachinidis, A.
Language:English
Date of Publication (YYYY-MM-DD):2010-03
Title of Journal:Genes to Cells
Volume:2010
Issue / Number:3
Start Page:209
End Page:228
Copyright:© 2010 John Wiley & Sons, Inc.
Review Status:not specified
Audience:Experts Only
Abstract / Description:Brachyury(+) mesodermal cell population with purity over 79% was obtained from differentiating brachyury embryonic stem cells (ESC) generated with brachyury promoter driven enhanced green fluorescent protein and puromycin-N-acetyltransferase. A comprehensive transcriptomic analysis of brachyury(+) cells enriched with puromycin application from 6-day-old embryoid bodies (EBs), 6-day-old control EBs and undifferentiated ESCs led to identification of 1573 uniquely up-regulated and 1549 uniquely down-regulated transcripts in brachyury(+) cells. Furthermore, transcripts up-regulated in brachyury(+) cells have overrepresented the Gene Ontology annotations (cell differentiation, blood vessel morphogenesis, striated muscle development, placenta development and cell motility) and Kyoto Encyclopedia of Genes and Genomes pathway annotations (mitogen-activated protein kinase signaling and transforming growth factor beta signaling). Transcripts representing Larp2 and Ankrd34b are notably up-regulated in brachyury(+) cells. Knockdown of Larp2 resulted in a significantly down-regulation BMP-2 expression, and knockdown of Ankrd34b resulted in alteration of NF-H, PPARgamma and PECAM1 expression. The elucidation of transcriptomic signatures of ESCs-derived brachyury(+) cells will contribute toward defining the genetic and cellular identities of presumptive mesodermal cells. Furthermore, there is a possible involvement of Larp2 in the regulation of the late mesodermal marker BMP-2. Ankrd34b might be a positive regulator of neurogenesis and a negative regulator of adipogenesis.
Comment of the Author/Creator:E-mail: a.sachinidis@uni-koeln.de
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:Center of Physiology and Pathophysiology, Institute of Neurophysiology, and Center of Molecular Medicine, University of Cologne (CMMC), Robert-Koch Str. 39, 50931 Cologne, Germany
Max-Delbrueck-Center for Molecular Medicine – MDC, Robert-Rössle Str. 10, 13092 Berlin, Germany
Institute of Computer Science, University of Tartu, Liivi 2, 50409 Tartu, Estonia and Quretec Ltd, Ulikooli 6a, Tartu, Estonia
Identifiers:ISSN:1365-2443 (Electronic) [ID No:1]
DOI:10.1111/j.1365-2443.2010.01390.x [ID No:2]
Full Text:
You have privileges to view the following file(s):
Doss.pdf  [1,00 Mb]   
 
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.