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| ID:
552731.0,
MPI für molekulare Genetik / Department of Vertebrate Genomics |
| Global transcriptomic analysis of murine embryonic stem cell-derived brachyury (T) cells |
| Authors: | Doss, M. X.; Wagh, V.; Schulz, H.; Kull, M.; Kolde, R.; Pfannkuche, K.; Nolden, T.; Himmelbauer, H.; Vilo, J.; Hescheler, J.; Sachinidis, A. | | Language: | English | | Date of Publication (YYYY-MM-DD): | 2010-03 | | Title of Journal: | Genes to Cells | | Volume: | 2010 | | Issue / Number: | 3 | | Start Page: | 209 | | End Page: | 228 | | Copyright: | © 2010 John Wiley & Sons, Inc. | | Review Status: | not specified | | Audience: | Experts Only | | Abstract / Description: | Brachyury(+) mesodermal cell population with purity over 79% was obtained from differentiating brachyury embryonic stem cells (ESC) generated with brachyury promoter driven enhanced green fluorescent protein and puromycin-N-acetyltransferase. A comprehensive transcriptomic analysis of brachyury(+) cells enriched with puromycin application from 6-day-old embryoid bodies (EBs), 6-day-old control EBs and undifferentiated ESCs led to identification of 1573 uniquely up-regulated and 1549 uniquely down-regulated transcripts in brachyury(+) cells. Furthermore, transcripts up-regulated in brachyury(+) cells have overrepresented the Gene Ontology annotations (cell differentiation, blood vessel morphogenesis, striated muscle development, placenta development and cell motility) and Kyoto Encyclopedia of Genes and Genomes pathway annotations (mitogen-activated protein kinase signaling and transforming growth factor beta signaling). Transcripts representing Larp2 and Ankrd34b are notably up-regulated in brachyury(+) cells. Knockdown of Larp2 resulted in a significantly down-regulation BMP-2 expression, and knockdown of Ankrd34b resulted in alteration of NF-H, PPARgamma and PECAM1 expression. The elucidation of transcriptomic signatures of ESCs-derived brachyury(+) cells will contribute toward defining the genetic and cellular identities of presumptive mesodermal cells. Furthermore, there is a possible involvement of Larp2 in the regulation of the late mesodermal marker BMP-2. Ankrd34b might be a positive regulator of neurogenesis and a negative regulator of adipogenesis. | | Comment of the Author/Creator: | E-mail: a.sachinidis@uni-koeln.de | | External Publication Status: | published | | Document Type: | Article | | Version Comment: | Automatic journal name synchronization |
| Communicated by: | Hans Lehrach | | Affiliations: | MPI für molekulare Genetik
| | External Affiliations: | Center of Physiology and Pathophysiology, Institute of Neurophysiology, and Center of Molecular Medicine, University of Cologne (CMMC), Robert-Koch Str. 39, 50931 Cologne, Germany
Max-Delbrueck-Center for Molecular Medicine – MDC, Robert-Rössle Str. 10, 13092 Berlin, Germany
Institute of Computer Science, University of Tartu, Liivi 2, 50409 Tartu, Estonia and Quretec Ltd, Ulikooli 6a, Tartu, Estonia
| | Identifiers: | ISSN:1365-2443 (Electronic) [ID No:1]
DOI:10.1111/j.1365-2443.2010.01390.x [ID No:2] |
| Full Text: |
| You have privileges to view the following file(s): |
| Doss.pdf [1,00 Mb] |
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