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          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents

ID: 552739.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
Mechanism of eIF6-mediated inhibition of ribosomal subunit joining
Authors:Gartmann, M.; Blau, M.; Armache, J. P.; Mielke, T.; Topf, M.; Beckmann, R.
Date of Publication (YYYY-MM-DD):2010-05-14
Title of Journal:The Journal of Biological Chemistry
Journal Abbrev.:J Biol Chem
Issue / Number:20
Start Page:14848
End Page:14851
Copyright:© 2010 by American Society for Biochemistry and Molecular Biology
Review Status:not specified
Audience:Experts Only
Abstract / Description:During the process of ribosomal assembly, the essential eukaryotic translation initiation factor 6 (eIF6) is known to act as a ribosomal anti-association factor. However, a molecular understanding of the anti-association activity of eIF6 is still missing. Here we present the cryo-electron microscopy reconstruction of a complex of the large ribosomal subunit with eukaryotic eIF6 from Saccharomyces cerevisiae. The structure reveals that the eIF6 binding site involves mainly rpL23 (L14p in Escherichia coli). Based on our structural data, we propose that the mechanism of the anti-association activity of eIF6 is based on steric hindrance of intersubunit bridge formation around the dynamic bridge B6.
Free Keywords:Microscopy, Electron; Models, Molecular; Peptide Initiation Factors/chemistry/physiology; Protein Conformation; Ribosomes/physiology; Saccharomyces cerevisiae Proteins/physiology
Comment of the Author/Creator:E-mail: beckmann@lmb.uni-muenchen.de
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:Department of Biochemistry, Gene Center and Center for Integrated Protein Science (CiPSM), Ludwig-Maximilians-Universität München, Feodor-Lynen-Strasse 25, 81377 Munich, Germany
Institut für Medizinische Physik und Biophysik, Charité, Ziegelstrasse 5-9, 10098 Berlin, Germany
Institut für Biochemie der Charité, Humboldt Universität Berlin, Monbijoustrasse 2, 10117 Berlin, Germany
Institute of Structural and Molecular Biology, School of Crystallography, Birkbeck College, University of London, Malet Street, London WC1E 7HX, United Kingdom
Identifiers:ISSN:1083-351X (Electronic) [ID No:1]
DOI:10.1074/jbc.C109.096057 [ID No:2]
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