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          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents

ID: 552744.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
A blueprint of ectoine metabolism from the genome of the industrial producer Halomonas elongata DSM 2581(T).
Authors:Schwibbert, K.; Marin-Sanguino, A.; Bagyan, I.; Heidrich, G.; Lentzen, G.; Seitz, H.; Rampp, M.; Schuster, S. C.; Klenk, H. P.; Pfeiffer, F.; Oesterhelt, D.; Kunte, H. J.
Date of Publication (YYYY-MM-DD):2010-09-16
Title of Journal:Environmental Microbiology
Journal Abbrev.:Environ Microbiol
Copyright:© 2010 Society for Applied Microbiology and Blackwell Publishing Ltd
Review Status:not specified
Audience:Experts Only
Abstract / Description:The halophilic gamma-proteobacterium Halomonas elongata DSM 2581(T) thrives at high salinity by synthesizing and accumulating the compatible solute ectoine. Ectoine levels are highly regulated according to external salt levels but the overall picture of its metabolism and control is not well understood. Apart from its critical role in cell adaptation to halophilic environments, ectoine can be used as a stabilizer for enzymes and as a cell protectant in skin and health care applications and is thus produced annually on a scale of tons in an industrial process using H. elongata as producer strain. This paper presents the complete genome sequence of H. elongata (4 061 296 bp) and includes experiments and analysis identifying and characterizing the entire ectoine metabolism, including a newly discovered pathway for ectoine degradation and its cyclic connection to ectoine synthesis. The degradation of ectoine (doe) proceeds via hydrolysis of ectoine (DoeA) to Nalpha-acetyl-l-2,4-diaminobutyric acid, followed by deacetylation to diaminobutyric acid (DoeB). In H. elongata, diaminobutyric acid can either flow off to aspartate or re-enter the ectoine synthesis pathway, forming a cycle of ectoine synthesis and degradation. Genome comparison revealed that the ectoine degradation pathway exists predominantly in non-halophilic bacteria unable to synthesize ectoine. Based on the resulting genetic and biochemical data, a metabolic flux model of ectoine metabolism was derived that can be used to understand the way H. elongata survives under varying salt stresses and that provides a basis for a model-driven improvement of industrial ectoine production.
Comment of the Author/Creator:Correspondence: E-mail hans-joerg.kunte@bam.de;
Tel. (+49) 30 8104 1414; Fax (+49) 30 8104 1417.
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
MPI für Plasmaphysik/Computer Center Garching (RZG)
MPI für Biochemie/Membrane and Neurophysics (P. Fromherz)
External Affiliations:1.Materials and Environment Division, Federal Institute for Materials Research and Testing (BAM), Berlin, Germany;
2.Department of Membrane Biochemistry, Max Planck Institute of Biochemistry, Martinsried, Germany;
3.Research and Development Division, Bitop AG, Witten, Germany;
4.Computing Center (RZG) of the Max-Planck-Society, Max Planck Institute of Plasma Physics, Garching, Germany;
5.Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, Pennsylvania, USA;
6.Microbiology, German Collection of Microorganisms and Cell Cultures (DSMZ), Braunschweig, Germany.
Identifiers:ISSN:1462-2912 [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=... [ID No:2]
DOI:10.1111/j.1462-2920.2010.02336.x. [ID No:3]
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