Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents



  history
ID: 553212.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
A streamlined protocol for emulsion polymerase chain reaction and subsequent purification.
Authors:Schütze, T.; Rubelt, F.; Repkow, J.; Greiner, N.; Erdmann, V. A.; Lehrach, H.; Konthur, Z.; Glokler, J.
Language:English
Research Context:This work was partially supported by the European Union through the EFRE Program (ProFIT Grant, Contract No. 10139409) and by the German Ministry of Science and Education (BMBF, Grant 0315448B).
Date of Publication (YYYY-MM-DD):2010-03-01
Title of Journal:Analytical Biochemistry
Journal Abbrev.:Anal Biochem
Volume:410
Issue / Number:1
Start Page:155
End Page:157
Copyright:© 2010 Elsevier Inc. All rights reserved.
Review Status:not specified
Audience:Experts Only
Abstract / Description:Compartmentalization of polymerase chain reaction (PCR) reduces artifacts, especially when complex libraries are amplified. It allows clonal amplification of templates from complex mixtures in a bias-free manner. Here we describe a rapid, straightforward, and easy protocol for PCR in a water-in-oil emulsion (ePCR) including sample recovery by DNA purification. Furthermore, no special laboratory equipment is needed and inexpensive components are used. Therefore, our flexible protocol allows ePCR to be readily implemented in daily routine experiments for a broad range of applications.
Comment of the Author/Creator:gloekler@molgen.mpg.de
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:1.Institute for Chemistry/Biochemistry, Freie Universität Berlin, Thielallee 63, 14195 Berlin, Germany.
Identifiers:ISSN:0003-2697 [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=... [ID No:2]
DOI:10.1016/j.ab.2010.11.029 [ID No:3]
Full Text:
You have privileges to view the following file(s):
Schütze_sdarticle.pdf  [468,00 Kb]   
 
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.