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          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents



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ID: 554579.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
Evaluation of the LightCycler(R) 1536 Instrument for high-throughput quantitative real-time PCR
Authors:Schlesinger, J.; Tönjes, M.; Schueler, M.; Zhang, Q.; Dunkel, I.; Sperling, S. R.
Research Context:This work was supported by the European Community’s Sixth Framework Program contract (“HeartRepair”) LSHM-CT-2005-018630 and European Community’s Seven Framework Program contract (“CardioGeNet”) 2009-223463, and a Ph.D. scholarship to Q.Z. by the German Academic Exchange Service (DAAD).
Date of Publication (YYYY-MM-DD):2010-03-07
Title of Journal:Methods
Journal Abbrev.:Methods
Volume:50
Issue / Number:4
Start Page:S19
End Page:S22
Copyright:© 2010 Elsevier Inc. All rights reserved.
Review Status:not specified
Audience:Experts Only
Abstract / Description:Quantitative real-time PCR (qPCR) is a frequently used, sensitive and accurate method to study gene expression profiles. However, its throughput was so far limited for routine laboratories to 384 reactions per run based on the limitations of the available instruments. Recently, the LightCycler(R) 1536 Instrument was launched providing a high-throughput solution for qPCR with the analysis of 1536 reactions in approximately 45min. We assessed the accuracy and sensitivity of this novel technology for the analysis of gene expression profiles in combination with the Innovadyne Nanodrop Express pipetting robot. We compared expression profiles obtained for 42 genes in 71 samples between the Universal ProbeLibrary and the LightCycler(R) 1536 Instrument and SYBR Green I and the ABI PRISM 7900HT system. We found that the results were highly reproducible between both systems. Beside the higher throughput, the advantage of the LightCycler(R) 1536 Instrument was the reduced consumption of reagents and sample material.
Free Keywords:LightCycler® 1536;
Hydrolysis probe assays;
Gene expression;
Real-time PCR;
Quantitative real-time PCR
Comment of the Author/Creator:Email:sperling@molgen.mpg.de
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
Identifiers:ISSN:1046-2023 [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=... [ID No:2]
DOI:10.1016/j.ymeth.2010.01.007 [ID No:2]
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