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ID: 554602.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
Proteomic and functional analysis of the mitotic Drosophila centrosome.
Authors:Müller, H.; Schmidt, D.; Steinbrink, S.; Mirgorodskaya, E.; Lehmann, V.; Habermann, K.; Dreher, F.; Gustavsson, N.; Kessler, T.; Lehrach, H.; Herwig, R.; Gobom, J.; Ploubidou, A.; Boutros, M.; Lange, B. M.
Language:English
Research Context:The work was funded by (i) BL laboratory: Berliner Senat für Kultur, Wissenschaft und Forschung, EFRE; NGFN2 SMP Protein; NGFN Plus, IG Mutanom; EU. (ii) MB laboratory: DFG, European Commission; HFSP. (iii) JG laboratory: Technologiestiftung Berlin (TSB); the Structural Fonds of the European Union within the project 2D/3D-ProteinChips, NGFN2 SMP Protein. Bruker Daltonics in Bremen is acknowledged for scientific collaboration. (iv) AP laboratory: Leibniz Association, Joint Initiative for Research and Innovation.
Date of Publication (YYYY-MM-DD):2010-10-06
Title of Journal:The EMBO Journal
Journal Abbrev.:EMBO J
Volume:29
Issue / Number:19
Start Page:3344
End Page:3357
Copyright:& 2010 European Molecular Biology Organization. All Rights Reserved
Review Status:not specified
Audience:Experts Only
Abstract / Description:Regulation of centrosome structure, duplication and segregation is integrated into cellular pathways that control cell cycle progression and growth. As part of these pathways, numerous proteins with well-established non-centrosomal localization and function associate with the centrosome to fulfill regulatory functions. In turn, classical centrosomal components take up functional and structural roles as part of other cellular organelles and compartments. Thus, although a comprehensive inventory of centrosome components is missing, emerging evidence indicates that its molecular composition reflects the complexity of its functions. We analysed the Drosophila embryonic centrosomal proteome using immunoisolation in combination with mass spectrometry. The 251 identified components were functionally characterized by RNA interference. Among those, a core group of 11 proteins was critical for centrosome structure maintenance. Depletion of any of these proteins in Drosophila SL2 cells resulted in centrosome disintegration, revealing a molecular dependency of centrosome structure on components of the protein translation machinery, actin- and RNA-binding proteins. In total, we assigned novel centrosome-related functions to 24 proteins and confirmed 13 of these in human cells.
Free Keywords:cell cycle;
centrosome;
Drosophila;
mitosis;
proteomics
Comment of the Author/Creator:Bodo M H Lange, Department of Vertebrate Genomics, Max-Planck Institute for Molecular Genetics, Ihnestr. 73, Berlin 14195, Germany. Tel.: +49 308 413 1645; Fax: +49 308 413 1128; E-mail: lange_b@molgen.mpg.de
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:1.Leibniz Institute for Age Research—Fritz Lipmann Institute, Jena, Germany;
2.German Cancer Research Center (DKFZ), Division of Signaling and Functional Genomics and University of Heidelberg, Faculty of Medicine Mannheim, Department of Cell and Molecular Biology, Heidelberg, Germany.
Identifiers:ISSN:0261-4189 [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=... [ID No:2]
DOI:10.1038/emboj.2010.210 [ID No:3]
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