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          Institute: MPI für molekulare Genetik     Collection: Department of Vertebrate Genomics     Display Documents



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ID: 555355.0, MPI für molekulare Genetik / Department of Vertebrate Genomics
Membrane attack complex inhibitor CD59a protects against focal cerebral ischemia in mice
Authors:Harhausen, D.; Khojasteh, U.; Stahel, P. F.; Morgan, B. P.; Nietfeld, W.; Dirnagl, U.; Trendelenburg, G.
Language:English
Date of Publication (YYYY-MM-DD):2010
Title of Journal:Journal of Neuroinflammation
Journal Abbrev.:J Neuroinflammation
Volume:7
Start Page:7:15
End Page:7:15
Copyright:© 2010 Harhausen et al; licensee BioMed Central Ltd
Review Status:not specified
Audience:Experts Only
Abstract / Description:BACKGROUND: The complement system is a crucial mediator of inflammation and cell lysis after cerebral ischemia. However, there is little information about the exact contribution of the membrane attack complex (MAC) and its inhibitor-protein CD59. METHODS: Transient focal cerebral ischemia was induced by middle cerebral artery occlusion (MCAO) in young male and female CD59a knockout and wild-type mice. Two models of MCAO were applied: 60 min MCAO and 48 h reperfusion, as well as 30 min MCAO and 72 h reperfusion. CD59a knockout animals were compared to wild-type animals in terms of infarct size, edema, neurological deficit, and cell death. RESULTS AND DISCUSSION: CD59a-deficiency in male mice caused significantly increased infarct volumes and brain swelling when compared to wild-type mice at 72 h after 30 min-occlusion time, whereas no significant difference was observed after 1 h-MCAO. Moreover, CD59a-deficient mice had impaired neurological function when compared to wild-type mice after 30 min MCAO. CONCLUSION: We conclude that CD59a protects against ischemic brain damage, but depending on the gender and the stroke model used.
Free Keywords:Animals; Antigens, CD11b/metabolism; Antigens, CD59/genetics/metabolism; Brain Infarction/etiology/metabolism/pathology; Disease Models, Animal; Female; In Situ Nick-End Labeling/methods; Infarction, Middle Cerebral Artery/complications/genetics/metabolism; Male; Mice; Mice, Inbred C57BL; Mice, Knockout; Neurologic Examination/methods; Sex Factors; Time Factors
External Publication Status:published
Document Type:Article
Communicated by:Hans Lehrach
Affiliations:MPI für molekulare Genetik
External Affiliations:Experimentelle Neurologie, Charité-Universitätsmedizin Berlin, CCM, 10117, Berlin, Germany
Dept. of Orthopaedic Surgery and Dept. of Neurosurgery,
Denver Health Medical Center, University of Colorado School of Medicine, 777 Bannock Street, CO, 80204 Denver, USA Department of Infection, Immunity and Biochemistry, School of Medicine, Cardiff University, Cardiff CF14 4XN, UK
Identifiers:ISSN:1742-2094 (Electronic) [ID No:1]
DOI:10.1186/1742-2094-7-15 [ID No:2]
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