Home News About Us Contact Contributors Disclaimer Privacy Policy Help FAQ

Home
Search
Quick Search
Advanced
Fulltext
Browse
Collections
Persons
My eDoc
Session History
Login
Name:
Password:
Documentation
Help
Support Wiki
Direct access to
document ID:


          Institute: MPI für Evolutionsbiologie     Collection: Evolutionary genetics     Display Documents



  history
ID: 557046.0, MPI für Evolutionsbiologie / Evolutionary genetics
General lack of global dosage compensation in ZZ/ZW systems? Broadening the perspective with RNA-seq
Authors:Wolf, Jochen B. W.; Bryk, Jarosłav
Language:English
Date of Publication (YYYY-MM-DD):2011-02-01
Title of Journal:Biomedcentral Genomics
Journal Abbrev.:BMC Genomics
Volume:12
Sequence Number of Article:91
Review Status:not specified
Audience:Not Specified
Abstract / Description:Background: Species with heteromorphic sex chromosomes face the challenge of large-scale imbalance in gene dose. Microarray-based studies in several independent male heterogametic XX/XY systems suggest that dosage compensation mechanisms are in place to mitigate the detrimental effects of gene dose differences. However, recent genomic research on female heterogametic ZZ/ZW systems has generated surprising results. In two bird species and one lepidopteran no evidence for a global dosage compensating mechanism has been found. The recent advent of massively parallel RNA sequencing now opens up the possibility to gauge the generality of this observation with a broader phylogenetic sampling. It further allows assessing the validity of microarray-based inference on dosage compensation with a novel technology.
Results: We here expemplify this approach using massively parallel sequencing on barcoded individuals of a bird species, the European crow (Corvus corone), where previously no genetic resources were available. Testing for Z-linkage with quantitative PCR (qPCR,) we first establish that orthology with distantly related species (chicken, zebra finch) can be used as a good predictor for chromosomal affiliation of a gene. We then use a digital measure of gene expression (RNA-seq) on brain transcriptome and confirm a global lack of dosage compensation on the Z chromosome. RNA-seq estimates of male-to-female (m:f) expression difference on the Z compare well to previous microarray-based estimates in birds and lepidopterans. The data further lends support that an up-regulation of female Z-linked genes conveys partial compensation and suggest a relationship between sex-bias and absolute expression level of a gene. Correlation of sex-biased gene expression on the Z chromosome across all three bird species further suggests that the degree of compensation has been partly conserved across 100 million years of avian evolution.
Conclusions: This work demonstrates that the study of dosage compensation has become amenable to species where previously no genetic resources were available. Massively parallele transcriptome sequencing allows reassessing the degree of dosage compensation with a novel tool in well-studies species and, in addition, gain valuable insights into the generality of mechanisms across independent taxonomic group for both the XX/XY and ZZ/ZW system.
External Publication Status:published
Document Type:Article
Communicated by:Brigitte Lechner
Affiliations:MPI für Evolutionsbiologie/Abt. Evolutionsgenetik
External Affiliations:Evolutionary Biology Centre, department of Evolutionary Biology, Uppsala University, Norbyvägen 18 D, 75236 Uppsala, Sweden
Identifiers:ISSN:1471-2164 [ID-No:1]
DOI:10.1186/1471-2164-12-91 [ID-No:2]
LOCALID:2828/S 39171 [Listen-Nummer/S-Nummer]
Full Text:
You have privileges to view the following file(s):
Wolf_2011b.pdf  [260,00 Kb]   
 
The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.