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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Publikationen des W. G. Kerckhoff-Instituts     Display Documents

ID: 559586.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Publikationen des W. G. Kerckhoff-Instituts
Electrostatic cell-surface repulsion initiates lumen formation in developing blood vessels
Authors:Strilic, B.; Eglinger, J.; Krieg, M.; Zeeb, M.; Axnick, J.; Babal, P.; Muller, D. J.; Lammert, E.
Title of Journal:Curr Biol
Issue / Number:22
Start Page:2003
End Page:9
Audience:Not Specified
Abstract / Description:Blood vessels function in the uptake, transport, and delivery of gases and nutrients within the body. A key question is how the central lumen of blood vessels develops within a cord of vascular endothelial cells. Here, we demonstrate that sialic acids of apical glycoproteins localize to apposing endothelial cell surfaces and generate repelling electrostatic fields within an endothelial cell cord. Both in vitro and in vivo experiments show that the negative charge of sialic acids is required for the separation of endothelial cell surfaces and subsequent lumen formation. We also demonstrate that sulfate residues can substitute for sialic acids during lumen initiation. These results therefore reveal a key step in the creation of blood vessels, the most abundant conduits in the vertebrate body. Because negatively charged mucins and proteoglycans are often found on luminal cell surfaces, it is possible that electrostatic repulsion is a general principle also used to initiate lumen formation in other organs.
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für physiologische und klinische Forschung
External Affiliations:Institute of Metabolic Physiology, Heinrich-Heine-University of Dusseldorf, D-40225 Dusseldorf, Germany.
Identifiers:ISSN:1879-0445 (Electronic) 0960-9822 (Linking)
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