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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Publikationen des W. G. Kerckhoff-Instituts     Display Documents

ID: 559615.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Publikationen des W. G. Kerckhoff-Instituts
Transcriptomic "portraits" of canine mammary cancer cell lines with various phenotypes
Authors:Krol, M.; Pawlowski, K. M.; Skierski, J.; Turowski, P.; Majewska, A.; Polanska, J.; Ugorski, M.; Morty, R. E.; Motyl, T.
Title of Journal:J Appl Genet
Issue / Number:2
Start Page:169
End Page:83
Audience:Not Specified
Abstract / Description:In light of the high incidence of mammary cancer in dogs and completion of the canine genome sequencing, the new possibilities of gene profiling by using DNA microarrays give hope to veterinary oncology. The cell lines isolated from mammary tumors are a valuable tool in developing and testing new pathway-specific cancer therapeutics. Differential cytometric analysis of 6 canine mammary cancer cell lines was performed. We divided cell lines into 3 groups based on their phenotype: 2 lines with high proliferative potential, 2 lines with high antiapoptotic potential, and 2 lines with high metastatic potential. DNA microarray analysis revealed common genes for cell lines of each group. We found that genes encoding the receptors for growth hormone and ghrelin are related to high proliferation rate, while ABR (active BCR-related) and TMD1 (TM2 domain containing 1) genes are related to a high antiapoptotic potential of the cancer cells. Metastatic properties of mammary cancer cells seem to be associated with elevated expression of PGP (P glycoprotein), SEMA3B (semaphorin 3B), and STIM1 (stromal interaction molecule 1).
Free Keywords:Adenocarcinoma/genetics/pathology/veterinary; Animals; Cell Division; Cell Line, Tumor; DNA, Neoplasm/genetics; Dog Diseases/*genetics; Dogs; Female; *Gene Expression Profiling; Kinetics; Mammary Neoplasms, Animal/*genetics/pathology; Oligonucleotide Array Sequence Analysis; Phenotype; Ploidies
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für physiologische und klinische Forschung
External Affiliations:Department of Physiological Sciences, Faculty of Veterinary Medicine, Warsaw University of Life Sciences (SGGW), Warsaw, Poland.
Identifiers:ISSN:1234-1983 (Print) 1234-1983 (Linking)
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