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          Institute: MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut)     Collection: Publikationen des W. G. Kerckhoff-Instituts     Display Documents

ID: 559779.0, MPI für Herz- und Lungenforschung (W. G. Kerckhoff Institut) / Publikationen des W. G. Kerckhoff-Instituts
Exercise linked to transient increase in expression and activity of cation channels in newly formed hind-limb collaterals
Authors:Sayed, A.; Schierling, W.; Troidl, K.; Ruding, I.; Nelson, K.; Apfelbeck, H.; Benli, I.; Schaper, W.; Schmitz-Rixen, T.
Title of Journal:Eur J Vasc Endovasc Surg
Issue / Number:1
Start Page:81
End Page:7
Audience:Not Specified
Abstract / Description:OBJECTIVE: This study aimed to compare arteriogenesis after femoral artery occlusion as influenced by exercise or arteriovenous shunt and follow changes in collateral transient receptor potential cation channel, subfamily V, member 4 (Trpv4). DESIGN: A prospective, controlled study wherein rats were subjected to femoral artery ligation (FAL), or FAL+arteriovenous shunt. Collateral Trpv4 was determined 0.5 and 6h post exercise. METHODS: Rats were subjected to exercise for 15 min, twice daily. The number and diameter of collaterals were assessed after 7 days. Collateral Trpv4 expression was quantified by reverse transcription-polymerase chain reaction. RESULTS: Collateral number and diameter per limb were significantly higher in the shunt group (number: 16.0+/-2.4 and diameter: 216.0+/-34 microm) compared to the ligature (number: 9.4+/-2 and diameter: 144+/-21 microm) and exercise groups (number: 9.9+/-2.5 and diameter: 151+/-15 microm). Trpv4 expression in collaterals harvested 0.5h post exercise was not significantly different from expression in shunted rats. It was significantly lower in collaterals harvested 6h post exercise (comparable to that in ligated rats). CONCLUSION: Collateral formation was greater in the shunt group than in the exercise group. Exercise-induced Trpv4 up-regulation, not significantly different from that achieved with shunt, returned to control values when evaluated 6h post exercise. More frequent exercise to chronically increase fluid shear stress, as with a shunt model, may be required for sufficient arteriogenesis to compensate for peripheral occlusion.
Free Keywords:Animals; Arterial Occlusive; Diseases/genetics/metabolism/*physiopathology/radiography; Arteries/metabolism/physiopathology; Arteriovenous Shunt, Surgical; *Collateral Circulation; Disease Models, Animal; Femoral Artery/surgery; Femoral Vein/surgery; Hindlimb; Male; Muscle, Skeletal/*blood supply; Neovascularization, Physiologic; *Physical Conditioning, Animal; RNA, Messenger/metabolism; Rats; Rats, Sprague-Dawley; TRPV Cation Channels/genetics/*metabolism; Time Factors; Up-Regulation
External Publication Status:published
Document Type:Article
Communicated by:N. N.
Affiliations:MPI für physiologische und klinische Forschung
External Affiliations:Department of General Surgery, Kasr Alainy Faculty of Medicine, Cairo University, Cairo, Egypt.
Identifiers:ISSN:1532-2165 (Electronic) 1078-5884 (Linking)
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