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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 1 - Protein Evolution (A. Lupas)     Display Documents

ID: 561531.0, MPI für Entwicklungsbiologie / Abteilung 1 - Protein Evolution (A. Lupas)
Cryo-EM structure and rRNA model of a translating eukaryotic 80S ribosome at 5.5-angstrom resolution
Authors:Armache, J. P.; Jarasch, A.; Anger, A. M.; Villa, E.; Becker, T.; Bhushan, S.; Jossinet, F.; Habeck, M.; Dindar, G.; Franckenberg, S.; Marquez, V.; Mielke, T.; Thomm, M.; Berninghausen, O.; Beatrix, B.; Soding, J.; Westhof, E.; Wilson, D. N.; Beckmann, R.
Date of Publication (YYYY-MM-DD):2010-11-16
Title of Journal:Proceedings of the National Academy of Sciences of the United States of America
Issue / Number:46
Start Page:19748
End Page:19753
Review Status:not specified
Audience:Not Specified
Abstract / Description:Protein biosynthesis, the translation of the genetic code into polypeptides, occurs on ribonucleoprotein particles called ribosomes. Although X-ray structures of bacterial ribosomes are available, high-resolution structures of eukaryotic 80S ribosomes are lacking. Using cryoelectron microscopy and single-particle reconstruction, we have determined the structure of a translating plant (Triticum aestivum) 80S ribosome at 5.5-angstrom resolution. This map, together with a 6.1-angstrom map of a Saccharomyces cerevisiae 80S ribosome, has enabled us to model similar to 98% of the rRNA. Accurate assignment of the rRNA expansion segments (ES) and variable regions has revealed unique ES-ES and r-protein-ES interactions, providing insight into the structure and evolution of the eukaryotic ribosome.
Free Keywords:modeling; molecular dynamics; flexible fitting; 5.5 angstrom resolution; electron-microscopy; molecular-dynamics; messenger-rna; rat-liver; secondary structure; expansion segments; cross-linking; 70s ribosome; exit tunnel
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 1 - Proteinevolution (Andrei Lupas)
External Affiliations:Univ Munich, Dept Biochem, Gene Ctr, Feodor Lynen Str 25, D-81377 Munich, Germany Univ Munich, Dept Biochem, Gene Ctr, Feodor Lynen Str 25, D-81377 Munich, Germany Univ Munich, Dept Biochem, Gene Ctr, D-81377 Munich, Germany Max Planck Inst Biochem, Dept Mol Struct Biol, D-82152 Martinsried, Germany Univ Strasbourg, Inst Biol Mol & Cellulaire, CNRS, F-67084 Strasbourg, France Max Planck Inst Biol Cybernet, Dept Empir Inference, D-72076 Tubingen, Germany Max Planck Inst Dev Biol, Dept Prot Evolut, D-72076 Tubingen, Germany Max Planck Inst Mol Genet, UltraStrukturNetzwerk, D-14195 Berlin, Germany Charite, Inst Med Phys & Biophys, D-10117 Berlin, Germany Univ Regensburg, Lehrstuhl Mikrobiol, D-93053 Regensburg, Germany Univ Munich, Dept Biochem, Ctr Integrated Prot Sci Munich, D-81377 Munich, Germany %G English
Identifiers:ISSN:0027-8424 %R DOI 10.1073/pnas.1009999107 [ID No:1]
ISI:000284261800030 [ID No:2]
ISI:000284261800030 [ID No:3]
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