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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 1 - Protein Evolution (A. Lupas)     Display Documents



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ID: 561532.0, MPI für Entwicklungsbiologie / Abteilung 1 - Protein Evolution (A. Lupas)
Localization of eukaryote-specific ribosomal proteins in a 5.5-angstrom cryo-EM map of the 80S eukaryotic ribosome
Authors:Armache, J. P.; Jarasch, A.; Anger, A. M.; Villa, E.; Becker, T.; Bhushan, S.; Jossinet, F.; Habeck, M.; Dindar, G.; Franckenberg, S.; Marquez, V.; Mielke, T.; Thomm, M.; Berninghausen, O.; Beatrix, B.; Soding, J.; Westhof, E.; Wilson, D. N.; Beckmann, R.
Date of Publication (YYYY-MM-DD):2010-11-16
Title of Journal:Proceedings of the National Academy of Sciences of the United States of America
Volume:107
Issue / Number:46
Start Page:19754
End Page:19759
Review Status:not specified
Audience:Not Specified
Abstract / Description:Protein synthesis in all living organisms occurs on ribonucleoprotein particles, called ribosomes. Despite the universality of this process, eukaryotic ribosomes are significantly larger in size than their bacterial counterparts due in part to the presence of 80 r proteins rather than 54 in bacteria. Using cryoelectron microscopy reconstructions of a translating plant (Triticum aestivum) 80S ribosome at 5.5-angstrom resolution, together with a 6.1-angstrom map of a translating Saccharomyces cerevisiae 80S ribosome, we have localized and modeled 74/80 (92.5%) of the ribosomal proteins, encompassing 12 archaeal/eukaryote-specific small subunit proteins as well as the complete complement of the ribosomal proteins of the eukaryotic large subunit. Near-complete atomic models of the 80S ribosome provide insights into the structure, function, and evolution of the eukaryotic translational apparatus.
Free Keywords:homology modeling; rna; translation; flexible fitting; molecular dynamics; multiple sequence alignment; 5.5 angstrom resolution; initiation-factor eif-3; virus ires rna; messenger-rna; cross-linking; saccharomyces-cerevisiae; translation initiation; structure prediction; spatial arrangement
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 1 - Proteinevolution (Andrei Lupas)
External Affiliations:Univ Munich, Dept Biochem, Gene Ctr, Feodor Lynen Str 25, D-81377 Munich, Germany Univ Munich, Dept Biochem, Gene Ctr, Feodor Lynen Str 25, D-81377 Munich, Germany Univ Munich, Dept Biochem, Gene Ctr, D-81377 Munich, Germany Univ Munich, Dept Biochem, Ctr Integrated Prot Sci Munich, D-81377 Munich, Germany Max Planck Inst Biochem, Dept Mol Struct Biol, D-82152 Martinsried, Germany Univ Strasbourg, Inst Biol Mol & Cellulaire, CNRS, F-67084 Strasbourg, France Max Planck Inst Biol Cybernet, Dept Empir Inference, D-72076 Tubingen, Germany Max Planck Inst Dev Biol, Dept Prot Evolut, D-72076 Tubingen, Germany Max Planck Inst Mol Genet, UltraStrukturNetzwerk, D-14195 Berlin, Germany Charite, Inst Med Phys & Biophys, D-10117 Berlin, Germany Univ Regensburg, Lehrstuhl Mikrobiol, D-93053 Regensburg, Germany %G English
Identifiers:ISSN:0027-8424 %R DOI 10.1073/pnas.1010005107 [ID No:1]
ISI:000284261800031 [ID No:2]
ISI:000284261800031 [ID No:3]
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