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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 1 - Protein Evolution (A. Lupas)     Display Documents

ID: 561541.0, MPI für Entwicklungsbiologie / Abteilung 1 - Protein Evolution (A. Lupas)
The 3D structures of VDAC represent a native conformation
Authors:Hiller, S.; Abramson, J.; Mannella, C.; Wagner, G.; Zeth, K.
Date of Publication (YYYY-MM-DD):2010-09
Title of Journal:Trends in Biochemical Sciences
Issue / Number:9
Start Page:514
End Page:521
Review Status:not specified
Audience:Not Specified
Abstract / Description:The most abundant protein of the mitochondrial outer membrane is the voltage-dependent anion channel (VDAC), which facilitates the exchange of ions and molecules between mitochondria and cytosol and is regulated by interactions with other proteins and small molecules. VDAC has been studied extensively for more than three decades, and last year three independent investigations revealed a structure of VDAC-1 exhibiting 19 transmembrane beta-strands, constituting a unique structural class of beta-barrel membrane proteins. Here, we provide a historical perspective on VDAC research and give an overview of the experimental design used to obtain these structures. Furthermore, we validate the protein refolding approach and summarize the biochemical and biophysical evidence that links the 19-stranded structure to the native form of VDAC.
Free Keywords:mitochondrial outer-membrane; dependent anion channel; permeability transition pore; selective channel; crystal-structure; escherichia-coli; neisseria-meningitidis; plant-mitochondria; creatine-kinase; ion channel
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 1 - Proteinevolution (Andrei Lupas)
External Affiliations:Univ Calif Los Angeles, Dept Physiol, Los Angeles, CA 90095 USA Univ Calif Los Angeles, Dept Physiol, Los Angeles, CA 90095 USA Harvard Univ, Sch Med, Dept Biol Chem & Mol Pharmacol, Boston, MA 02115 USA New York State Dept Hlth, Wadsworth Ctr, Albany, NY 12201 USA Max Planck Inst Dev Biol, Dept Prot Evolut, D-72076 Tubingen, Germany %G English
Identifiers:ISSN:0968-0004 [ID No:1]
ISI:000281913500006 [ID No:2]
ISI:000281913500006 [ID No:3]
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