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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilungsunabhängige Arbeitsgruppen     Display Documents

ID: 561569.0, MPI für Entwicklungsbiologie / Abteilungsunabhängige Arbeitsgruppen
Dynamic Regulation of Archaeal Proteasome Gate Opening As Studied by TROSY NMR
Authors:Religa, T. L.; Sprangers, R.; Kay, L. E.
Date of Publication (YYYY-MM-DD):2010-04-02
Title of Journal:Science
Issue / Number:5974
Start Page:98
End Page:102
Review Status:not specified
Audience:Not Specified
Abstract / Description:The proteasome catalyzes the majority of protein degradation in the cell and plays an integral role in cellular homeostasis. Control over proteolysis by the 20S core-particle (CP) proteasome is achieved by gated access of substrate; thus, an understanding of the molecular mechanism by which these gates regulate substrate entry is critical. We used methyl-transverse relaxation optimized nuclear magnetic resonance spectroscopy to show that the amino-terminal residues that compose the gates of the a subunits of the Thermoplasma acidophilum proteasome are highly dynamic over a broad spectrum of time scales and that gating termini are in conformations that extend either well inside (closed gate) or outside (open gate) of the antechamber. Interconversion between these conformers on a time scale of seconds leads to a dynamic regulation of 20S CP proteolysis activity.
Free Keywords:20s proteasome; protein-degradation; structural basis; relaxation; resolution; atpases; termini
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilungsunabhängige Arbeitsgruppen
External Affiliations:Univ Toronto, Dept Mol Genet, 100 Coll St, Toronto, ON M5S 1A8, Canada Univ Toronto, Dept Mol Genet, Toronto, ON M5S 1A8, Canada Univ Toronto, Dept Biochem, Toronto, ON M5S 1A8, Canada Univ Toronto, Dept Chem, Toronto, ON M5S 1A8, Canada Max Planck Inst Dev Biol, Tubingen, Germany %G English
Identifiers:ISSN:0036-8075 [ID No:1]
ISI:000276202200044 [ID No:2]
ISI:000276202200044 [ID No:3]
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