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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 6 - Molecular Biology (D. Weigel)     Display Documents



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ID: 561620.0, MPI für Entwicklungsbiologie / Abteilung 6 - Molecular Biology (D. Weigel)
Increased Leaf Size: Different Means to an End
Authors:Gonzalez, N.; De Bodt, S.; Sulpice, R.; Jikumaru, Y.; Chae, E.; Dhondt, S.; Van Daele, T.; De Milde, L.; Weigel, D.; Kamiya, Y.; Stitt, M.; Beemster, G. T. S.; Inze, D.
Date of Publication (YYYY-MM-DD):2010-07
Title of Journal:Plant Physiology
Volume:153
Issue / Number:3
Start Page:1261
End Page:1279
Review Status:not specified
Audience:Not Specified
Abstract / Description:The final size of plant organs, such as leaves, is tightly controlled by environmental and genetic factors that must spatially and temporally coordinate cell expansion and cell cycle activity. However, this regulation of organ growth is still poorly understood. The aim of this study is to gain more insight into the genetic control of leaf size in Arabidopsis (Arabidopsis thaliana) by performing a comparative analysis of transgenic lines that produce enlarged leaves under standardized environmental conditions. To this end, we selected five genes belonging to different functional classes that all positively affect leaf size when overexpressed: AVP1, GRF5, JAW, BRI1, and GA20OX1. We show that the increase in leaf area in these lines depended on leaf position and growth conditions and that all five lines affected leaf size differently; however, in all cases, an increase in cell number was, entirely or predominantly, responsible for the leaf size enlargement. By analyzing hormone levels, transcriptome, and metabolome, we provide deeper insight into the molecular basis of the growth phenotype for the individual lines. A comparative analysis between these data sets indicates that enhanced organ growth is governed by different, seemingly independent pathways. The analysis of transgenic lines simultaneously overexpressing two growth-enhancing genes further supports the concept that multiple pathways independently converge on organ size control in Arabidopsis.
Free Keywords:arabidopsis-thaliana; plant development; cell-proliferation; organ size; synovial sarcoma; growth; gene; expression; brassinosteroids; pyrophosphorylase
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 6 - Molekulare Biologie (Detlef Weigel)
External Affiliations:Univ Ghent VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium Univ Ghent VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium Univ Ghent VIB, Dept Plant Syst Biol, B-9052 Ghent, Belgium Univ Ghent, Dept Plant Biotechnol & Genet, B-9052 Ghent, Belgium Max Planck Inst Mol Plant Physiol, D-14476 Potsdam, Germany RIKEN, Plant Sci Ctr, Kanagawa 2300045, Japan Max Planck Inst Dev Biol, Dept Mol Biol, D-72076 Tubingen, Germany Univ Antwerp, Dept Biol, B-2020 Antwerp, Belgium %G English
Identifiers:ISSN:0032-0889 %R DOI 10.1104/pp.110.156018 [ID No:1]
ISI:000279400200030 [ID No:2]
ISI:000279400200030 [ID No:3]
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