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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 6 - Molecular Biology (D. Weigel)     Display Documents



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ID: 561622.0, MPI für Entwicklungsbiologie / Abteilung 6 - Molecular Biology (D. Weigel)
Transcriptional Control of Gene Expression by MicroRNAs
Authors:Khraiwesh, B.; Arif, M. A.; Seumel, G. I.; Ossowski, S.; Weigel, D.; Reski, R.; Frank, W.
Date of Publication (YYYY-MM-DD):2010-01-08
Title of Journal:Cell
Volume:140
Issue / Number:1
Start Page:111
End Page:122
Review Status:not specified
Audience:Not Specified
Abstract / Description:MicroRNAs (miRNAs) control gene expression in animals and plants. Like another class of small RNAs, siRNAs, they affect gene expression posttranscriptionally. While siRNAs in addition act in transcriptional gene silencing, a role of miRNAs in transcriptional regulation has been less clear. We show here that in moss Physcomitrella patens mutants without a DICER-LIKE1b gene, maturation of miRNAs is normal but cleavage of target RNAs is abolished and levels of these transcripts are drastically reduced. These mutants accumulate miRNA: target-RNA duplexes and show hypermethylation of the genes encoding target RNAs, leading to gene silencing. This pathway occurs also in the wild-type upon hormone treatment. We propose that initiation of epigenetic silencing by DNA methylation depends on the ratio of the miRNA and its target RNA.
Free Keywords:directed DNA methylation; dependent rna-polymerase; trans-acting sirnas; physcomitrella-patens; arabidopsis-thaliana; c-elegans; mammalian-cells; messenger-rnas; dicer homolog; plants
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 6 - Molekulare Biologie (Detlef Weigel)
External Affiliations:Univ Freiburg, Fac Biol, Schaenzlestr 1, D-79104 Freiburg, Germany Univ Freiburg, Fac Biol, Schaenzlestr 1, D-79104 Freiburg, Germany Univ Freiburg, Fac Biol, D-79104 Freiburg, Germany Max Planck Inst Dev Biol, Dept Mol Biol, D-72076 Tubingen, Germany Freiburg Initiat Syst Biol, D-79104 Freiburg, Germany Ctr Biol Signalling Studies, D-79104 Freiburg, Germany %G English
Identifiers:ISSN:0092-8674 %R DOI 10.1016/j.cell.2009.12.023 [ID No:1]
ISI:000273391900019 [ID No:2]
ISI:000273391900019 [ID No:3]
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