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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 6 - Molecular Biology (D. Weigel)     Display Documents



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ID: 561625.0, MPI für Entwicklungsbiologie / Abteilung 6 - Molecular Biology (D. Weigel)
Global effects of the small RNA biogenesis machinery on the Arabidopsis thaliana transcriptome
Authors:Laubinger, S.; Zeller, G.; Henz, S. R.; Buechel, S.; Sachsenberg, T.; Wang, J. W.; Ratsch, G.; Weigel, D.
Date of Publication (YYYY-MM-DD):2010-10-12
Title of Journal:Proceedings of the National Academy of Sciences of the United States of America
Volume:107
Issue / Number:41
Start Page:17466
End Page:17473
Review Status:not specified
Audience:Not Specified
Abstract / Description:In Arabidopsis thaliana, four different dicer-like (DCL) proteins have distinct but partially overlapping functions in the biogenesis of microRNAs (miRNAs) and siRNAs from longer, noncoding precursor RNAs. To analyze the impact of different components of the small RNA biogenesis machinery on the transcriptome, we subjected dcl and other mutants impaired in small RNA biogenesis to whole-genome tiling array analysis. We compared both protein-coding genes and noncoding transcripts, including most pri-miRNAs, in two tissues and several stress conditions. Our analysis revealed a surprising number of common targets in dcl1 and dcl2 dcl3 dcl4 triple mutants. Furthermore, our results suggest that the DCL1 is not only involved in miRNA action but also contributes to silencing of a subset of transposons, apparently through an effect on DNA methylation.
Free Keywords:Arabidopsis/genetics/*metabolism; Arabidopsis Proteins/genetics/*metabolism; Cell Cycle Proteins/genetics/*metabolism; DNA Methylation; DNA Transposable Elements/genetics; Gene Expression Profiling/methods; Gene Expression Regulation, Plant/genetics/*physiology; MicroRNAs/*biosynthesis; Mutation/genetics; Protein Array Analysis; Reverse Transcriptase Polymerase Chain Reaction; Ribonuclease III/genetics/*metabolism
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 6 - Molekulare Biologie (Detlef Weigel)
External Affiliations:%G eng
Identifiers:ISSN:1091-6490 (Electronic) 0027-8424 (Linking) %R 1012... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/20870966 [ID No:2]
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