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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 6 - Molecular Biology (D. Weigel)     Display Documents



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ID: 561628.0, MPI für Entwicklungsbiologie / Abteilung 6 - Molecular Biology (D. Weigel)
The rate and molecular spectrum of spontaneous mutations in Arabidopsis thaliana
Authors:Ossowski, S.; Schneeberger, K.; Lucas-Lledo, J. I.; Warthmann, N.; Clark, R. M.; Shaw, R. G.; Weigel, D.; Lynch, M.
Date of Publication (YYYY-MM-DD):2010-01-01
Title of Journal:Science
Volume:327
Issue / Number:5961
Start Page:92
End Page:94
Review Status:not specified
Audience:Not Specified
Abstract / Description:To take complete advantage of information on within-species polymorphism and divergence from close relatives, one needs to know the rate and the molecular spectrum of spontaneous mutations. To this end, we have searched for de novo spontaneous mutations in the complete nuclear genomes of five Arabidopsis thaliana mutation accumulation lines that had been maintained by single-seed descent for 30 generations. We identified and validated 99 base substitutions and 17 small and large insertions and deletions. Our results imply a spontaneous mutation rate of 7 x 10(-9) base substitutions per site per generation, the majority of which are G:C-->A:T transitions. We explain this very biased spectrum of base substitution mutations as a result of two main processes: deamination of methylated cytosines and ultraviolet light-induced mutagenesis.
Free Keywords:Arabidopsis/*genetics/radiation effects; Cytosine/metabolism; DNA Methylation; DNA, Intergenic; DNA, Plant/*genetics; Deamination; *Genome, Plant; INDEL Mutation; *Mutation; Sequence Analysis, DNA; Sequence Deletion; Ultraviolet Rays
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 6 - Molekulare Biologie (Detlef Weigel)
External Affiliations:%G eng
Identifiers:ISSN:1095-9203 (Electronic) 0036-8075 (Linking) %R 327/... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/20044577 [ID No:2]
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