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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 2 - Biochemistry (E. Izaurralde)     Display Documents

ID: 561655.0, MPI für Entwicklungsbiologie / Abteilung 2 - Biochemistry (E. Izaurralde)
HPat provides a link between deadenylation and decapping in metazoa
Authors:Haas, G.; Braun, J. E.; Igreja, C.; Tritschler, F.; Nishihara, T.; Izaurralde, E.
Date of Publication (YYYY-MM-DD):2010-04-19
Title of Journal:Journal of Cell Biology
Issue / Number:2
Start Page:289
End Page:302
Review Status:not specified
Audience:Not Specified
Abstract / Description:Decapping of eukaryotic messenger RNAs (mRNAs) occurs after they have undergone deadenylation, but how these processes are coordinated is poorly understood. In this study, we report that Drosophila melanogaster HPat (homologue of Pat1), a conserved decapping activator, interacts with additional decapping factors (e.g., Me31B, the LSm1-7 complex, and the decapping enzyme DCP2) and with components of the CCR4-NOT deadenylase complex. Accordingly, HPat triggers deadenylation and decapping when artificially tethered to an mRNA reporter. These activities reside, unexpectedly, in a proline-rich region. However, this region alone cannot restore decapping in cells depleted of endogenous HPat but also requires the middle (Mid) and the very C-terminal domains of HPat. We further show that the Mid and C-terminal domains mediate HPat recruitment to target mRNAs. Our results reveal an unprecedented role for the proline-rich region and the C-terminal domain of metazoan HPat in mRNA decapping and suggest that HPat is a component of the cellular mechanism that couples decapping to deadenylation in vivo.
Free Keywords:Amino Acid Sequence; Animals; Cells, Cultured; DEAD-box RNA Helicases/genetics/metabolism; Drosophila Proteins/genetics/*metabolism; Drosophila melanogaster/genetics/*metabolism; Endoribonucleases/genetics/metabolism; Homeodomain Proteins/genetics/metabolism; Humans; Molecular Sequence Data; Protein Structure, Tertiary; RNA Caps/*metabolism; RNA Stability; RNA, Messenger/genetics/*metabolism; RNA-Binding Proteins/genetics/*metabolism; Recombinant Fusion Proteins/genetics/metabolism; Ribonucleases/genetics/metabolism; Ribonucleoproteins, Small Nuclear/genetics/metabolism; Sequence Alignment; Transcription Factors/genetics/metabolism
External Publication Status:published
Document Type:Article
Communicated by:root
Affiliations:MPI für Entwicklungsbiologie/Abteilung 2 - Biochemie (Elisa Izaurralde)
External Affiliations:%G eng
Identifiers:ISSN:1540-8140 (Electronic) 0021-9525 (Linking) %R jcb.... [ID No:1]
URL: [ID No:2]
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