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          Institute: MPI für medizinische Forschung     Collection: Abteilung Biophysik     Display Documents



ID: 565718.0, MPI für medizinische Forschung / Abteilung Biophysik
Dictyostelium myosin−IE is a fast molecular motor involved in phagocytosis
Translation of Title:Dictyostelium myosin−IE is a fast molecular motor involved in phagocytosis
Authors:Dürrwang, Ulrike; Fujita−Becker, Setsuko; Erent, Muriel; Kull, F. Jon; Tsiavaliaris, Georgios; Geeves, Michael A.; Manstein, Dietmar J.
Language:English
Date of Publication (YYYY-MM-DD):2006-01-27
Title of Journal:Journal of Cell Science
Journal Abbrev.:J. Cell Sci.
Volume:119
Start Page:550
End Page:558
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Class I myosins are single−headed motor proteins, implicated in various motile processes including organelle translocation, ion−channel gating, and cytoskeleton reorganization. Here we describe the cellular localization of myosin−IE and its role in the phagocytic uptake of solid particles and cells. A complete analysis of the kinetic and motor properties of Dictyostelium discoideum myosin−IE was achieved by the use of motor domain constructs with artificial lever arms. Class I myosins belonging to subclass IC like myosin−IE are thought to be tuned for tension maintenance or stress sensing. In contrast to this prediction, our results show myosin−IE to be a fast motor. Myosin−IE motor activity is regulated by myosin heavy chain phosphorylation, which increases the coupling efficiency between the actin and nucleotide binding sites tenfold and the motile activity more than fivefold. Changes in the level of free Mg(2+) ions, which are within the physiological range, are shown to modulate the motor activity of myosin−IE by inhibiting the release of adenosine diphosphate
Free Keywords:Dictyostelium discoideum, Phagocytosis, Phosphorylation, TEDS, Mg2+ concentration
Last Change of the Resource (YYYY-MM-DD):--
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biophysik/Gruppe Dietmar Manstein
MPI für medizinische Forschung/Abteilung Biophysik/Muskelforschung
MPI für medizinische Forschung/Abteilung Biophysik
Identifiers:LOCALID:6805
URI:http%3A%2F%2Fjcs.biologists.org%2Fcgi%2Freprint%2F...
URI:http%3A%2F%2Fjcs.biologists.org%2Fcgi%2Fcontent%2F...
URI:http%3A%2F%2Fjcs.biologists.org%2Fcgi%2Fcontent%2F...
DOI:10.1242%2F10.1242%2Fjcs.02774
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