| ID:
566042.0,
MPI für medizinische Forschung / Kortikale Zwei - Photonen - Bildgebungsverfahren |
| Visually evoked activity in cortical cells imaged in freely moving animals |
| Translation of Title: | Visually evoked activity in cortical cells imaged in freely moving animals |
| Authors: | Sawinski, Jürgen; Haydon−Wallace, Damian J.; Greenberg, D. S.; Grossmann, Silvie; Denk, Winfried; Kerr, Jason |
| Language: | English |
| Date of Publication (YYYY-MM-DD): | 2009-11-17 |
| Title of Journal: | Proceedings of the National Academy of Sciences of the USA (JSTOR) |
| Journal Abbrev.: | Proc. Natl. Acad. Sci. U. S. A. |
| Volume: | 106 |
| Issue / Number: | 46 |
| Start Page: | 19557 |
| End Page: | 19562 |
| Review Status: | Peer-review |
| Audience: | Experts Only |
| Intended Educational Use: | No |
| Abstract / Description: | We describe a miniaturized head−mounted multiphoton microscope
and its use for recording Ca2 transients from the somata of
layer 2/3 neurons in the visual cortex of awake, freely moving rats.
Images contained up to 20 neurons and were stable enough to
record continuously for >5 min per trial and 20 trials per imaging
session, even as the animal was running at velocities of up to 0.6
m/s. Neuronal Ca2 transients were readily detected, and responses
to various static visual stimuli were observed during free
movement on a running track. Neuronal activity was sparse and
increased when the animal swept its gaze across a visual stimulus.
Neurons showing preferential activation by specific stimuli were
observed in freely moving animals. These results demonstrate that
the multiphoton fiberscope is suitable for functional imaging in
awake and freely moving animals |
| Free Keywords: | calcium imaging, head−mounted microscope neuronal activity,
two−photon, visual cortex |
| Last Change of the Resource (YYYY-MM-DD): | -- |
| External Publication Status: | published |
| Document Type: | Article |
| Version Comment: | Automatic journal name synchronization |
| Communicated by: | Wulf Kaiser |
| Affiliations: | MPI für medizinische Forschung/Abteilung Zellphysiologie
MPI für medizinische Forschung/Abteilung Biomedizinische Optik
MPI für medizinische Forschung/Abteilung Zellphysiologie/Kortikale Zwei - Photonen - Bildgebungsverfahren
MPI für medizinische Forschung/Abteilung Zellphysiologie/Whisker Representation
MPI für medizinische Forschung/Abteilung Zellphysiologie/In Vivo - Mikroskopie kortikaler Aktivitäten
|
| Identifiers: | LOCALID:7560
URI:http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles...
URI:http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles...
URI:http%3A%2F%2Fwww.ncbi.nlm.nih.gov%2Fpmc%2Farticles...
DOI:10.1073%2Fpnas.0903680106 |
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