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          Institute: MPI für medizinische Forschung     Collection: Olfaction Web     Display Documents

ID: 566154.0, MPI für medizinische Forschung / Olfaction Web
Characterization of infectious and defective cloned avian hepadnavirus genomes
Translation of Title:Characterization of infectious and defective cloned avian hepadnavirus genomes
Authors:Wildner, Gerhild; Fernholz, Doris; Sprengel, Rolf; Schneider, Ralf; Will, Hans
Date of Publication (YYYY-MM-DD):1991-11-01
Title of Journal:Virology
Journal Abbrev.:Virology
Issue / Number:1
Start Page:345
End Page:353
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:The infectivity in vivo, replication competence in vitro, and expression of viral genes of several molecularly cloned duck hepatitis B virus (DHBV) genomes were investigated. In addition, replication competence, core protein expression, and secretion of viral proteins were investigated for a grey heron hepatitis B virus genome. Except two, all DHBV isolates tested induced a systemic infection in Pekin ducks when injected as cloned viral DNA into the liver. After transfection of chicken hepatoma cells, both defective DHBV genomes expressed intracellular nucleocapsid and pre−S envelope proteins and secreted DHBs/pre−S particles into the medium. One of the defective DHBV genomes and HHBV produced within the cells replicative intermediates encapsidated in core particles and secreted virions, whereas the other defective DHBV genome did not and was unable to efficiently encapsidate the RNA pregenome. Comparative sequence analysis was performed to identify potential amino acid changes in viral proteins of both defective DHBV genomes. The data obtained demonstrate that most cloned avian hepadnaviruses are infectious or replication competent and suggest defects in envelope, polymerase or encapsidation function, respectively, in two cloned DHBV genomes
Last Change of the Resource (YYYY-MM-DD):--
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie
MPI für medizinische Forschung/Abteilung Zellphysiologie/Olfaction Web
MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie/Gruppe Rolf Sprengel
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