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          Institute: MPI für medizinische Forschung     Collection: Abteilung Molekulare Neurobiologie     Display Documents

ID: 566583.0, MPI für medizinische Forschung / Abteilung Molekulare Neurobiologie
Proton Magnetic Resonance Studies of Cholinergic Ligand Binding to the Acetylcholine Receptor in its Membrane Environment
Translation of Title:Proton Magnetic Resonance Studies of Cholinergic Ligand Binding to the Acetylcholine Receptor in its Membrane Environment
Authors:Miller, James; Witzemann, Veit; Quast, Ulrich; Raftery, Michael A.
Date of Publication (YYYY-MM-DD):1979-08-01
Title of Journal:Proceedings of the National Academy of Sciences of the USA (JSTOR)
Journal Abbrev.:Proc. Natl. Acad. Sci. U. S. A.
Issue / Number:8
Start Page:3580
End Page:3584
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Proton magnetic resonance has been used to monitor binding of choline, a known partial agonist, to acetylcholine receptor−enriched membrane preparations from Torpedo californica electroplax. The interaction between choline and receptor led to a broadening of the resonance of the choline methyl groups and this effect was reversed by alpha −bungarotoxin, a quasi−irreversible antagonist of the acetylcholine receptor. From the concentration dependence of line broadening the equilibrium dissociation constant for choline was obtained (Kd = 190 ± 65 µ M). The temperature dependence of the parameters observed in the choline titrations gave an enthalpy of binding Delta H < 1.5 kcal/mol and allowed estimates for the dissociation rate constant of the receptor−choline complex (kdiss > 1.6 x 103 S1) and the respective activation energy, Ea (kdiss) approx 5.5 kcal/mol. The association of other ligands with the membrane−bound receptor could also be studied by observing effects of varying concentrations of such ligands on the choline methyl group linewidth at a constant choline concentration
Last Change of the Resource (YYYY-MM-DD):--
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Zellphysiologie
MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie
MPI für medizinische Forschung/Abteilung Zellphysiologie/Molekulare Anatomie neuromuskulärer Synapsen
MPI für medizinische Forschung/Arbeitsgruppe Witzemann / Koenen
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