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          Institute: MPI für medizinische Forschung     Collection: Abteilung Molekulare Neurobiologie     Display Documents

ID: 566760.0, MPI für medizinische Forschung / Abteilung Molekulare Neurobiologie
Viral vectors: a wide range of choices and high levels of service
Translation of Title:Viral vectors: a wide range of choices and high levels of service
Authors:Osten, Pavel; Grinevich, Valery; Cetin, Ali
Date of Publication (YYYY-MM-DD):2007-01-01
Title of Journal:Handb. Exp. Pharmacol.
Journal Abbrev.:Handb. Exp. Pharmacol.
Start Page:177
End Page:202
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Viruses are intracellular parasites with simple DNA or RNA genomes. Virus life revolves around three steps: infection of a host cell, replication of its genome within the host cell environment, and formation of new virions; this process is often but not always associated with pathogenic effects against the host organism. Since the mid−1980s, the main goal of viral vectorology has been to develop recombinant viral vectors for long−term gene delivery to mammalian cells, with minimal associated toxicity. Today, several viral vector systems are close to achieving this aim, providing stable transgenic expression in many different cell types and tissues. Here we review application characteristics of four vector systems, derived from adeno−associated viruses, adenoviruses, retroviruses and herpes simplex virus−1, for in vivo gene delivery. We discuss the transfer capacity of the expression vectors, the stability of their transgenic expression, the tropism of the recombinant viruses, the likelihood of induction of immunotoxicity, and the ease (or difficulty) of the virus production. In the end, we discuss applications of these vectors for delivery of three molecular systems for conditional mutagenesis, two for inducible transcriptional control of transgenic expression (the tet and the dimerizer systems), and the third one for inducible control of endogenous gene expression based on RNA interference
Free Keywords:Recombinant viral vectors − Gene delivery − Conditional gene expression − Conditional gene knock−down
Last Change of the Resource (YYYY-MM-DD):--
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie
MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie/Gruppe Pavel Osten
MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie/Gruppe Valery Grinevich
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