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          Institute: MPI für medizinische Forschung     Collection: Gruppe Rolf Sprengel     Display Documents



ID: 566909.0, MPI für medizinische Forschung / Gruppe Rolf Sprengel
Homologous recombination between hepadnaviral genomes following in Vivo DNA transfection: Implications for studies of viral infectivity
Translation of Title:Homologous recombination between hepadnaviral genomes following in Vivo DNA transfection: Implications for studies of viral infectivity
Authors:Sprengel, Rolf; Varmus, Harold E.; Ganem, Don
Language:English
Date of Publication (YYYY-MM-DD):1987-08-01
Title of Journal:Virology
Journal Abbrev.:Virology
Volume:159
Issue / Number:2
Start Page:454
End Page:456
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:The cloned DNA of duck hepatitis B virus (DHBV) can initiate a productive infection of susceptible ducklings when presented by direct intrahepatic injection. We have examined the effects of the structure of the incoming DNA upon the outcome of this in vivo transfection. Plasmid−linked DHBV genomes of greater than unit length regularly give rise to infectious virus irrespective of the location of the plasmid insert on the transcriptional map. When mutant DHBV genomes are coinjected with subgenomic viral DNA fragments spanning the mutation, wild−type recombinants arise. These results indicate that, as in transfection of cultured cells, in vivo transfection of hepatocytes is regularly followed by homologous recombination involving incoming DNA molecules. These frequent recombination events will complicate efforts to use in vivo transfection for certain genetic analyses of hepadnaviruses
Last Change of the Resource (YYYY-MM-DD):--
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie
MPI für medizinische Forschung/Abteilung Zellphysiologie/Olfaction Web
MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie/Gruppe Rolf Sprengel
Identifiers:LOCALID:7369
URI:http%3A%2F%2Fwww.sciencedirect.com%2Fscience%3F_ob...
URI:http%3A%2F%2Fwww.sciencedirect.com%2Fscience%3F_ob...
DOI:doi%3A10.1016%2F0042-6822%2887%2990486-7%20%20%20%...
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