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          Institute: MPI für medizinische Forschung     Collection: Gruppe Pavel Osten     Display Documents

ID: 567015.0, MPI für medizinische Forschung / Gruppe Pavel Osten
Acute endotoxemia in rats induces down−regulation of V2 vasopressin receptors and aquaporin−2 content in the kidney medulla
Translation of Title:Acute endotoxemia in rats induces down−regulation of V2 vasopressin receptors and aquaporin−2 content in the kidney medulla
Authors:Grinevich, Valery; Knepper, Mark A.; Verbalis, Joseph; Reyes, Ivan; Aguilera, Greti
Date of Publication (YYYY-MM-DD):2004-01-01
Title of Journal:Kidney Int.
Journal Abbrev.:Kidney Int.
Issue / Number:1
Start Page:54
End Page:62
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Acute endotoxemia in rats induces down−regulation of V2 vasopressin receptors and aquaporin−2 content in the kidney medulla. Endotoxemia can lead to fluid metabolism alterations despite unchanged or elevated plasma vasopressin (VP) levels, suggesting a refractoriness of the kidney to the effect of the peptide. To test this hypothesis, we examined the effect of lipopolysaccharide (LPS) injection on the expression of V2 receptors and aquaporin−2 in the kidney. Plasma VP and urine osmolality, and binding of [3H]VP to kidney membranes, Western blot, and immunohistochemical analysis of aquaporin−2, in situ hybridization for V2 VP receptors and cytokines mRNAs were measured in the kidney 3 to 24 hours after LPS injection, 250 mug/100 g, intraperitoneally. LPS injection caused prolonged decreases in urine osmolality (up to 24 hours) without significant changes in plasma levels of sodium or VP. This was associated with marked decreases in V2 VP receptor mRNA and VP receptor number in the kidney, which were evident for up to 12 hours after LPS injection. Aquaporin−2 in kidney inner medulla was also reduced by about 50%. LPS induced interleukin (IL)−1beta in the kidney medulla by 3 hours, reached maximum at 6 hours, and started to decline by 12 hours, while it increased IL−6 mRNA significantly only at 3 hours. Interleukin mRNA expression was absent in kidneys of control rats. In vitro incubation of kidney medulla slices with IL−1beta reduced VP binding. The inflammatory response to acute endotoxemia down regulates V2 VP receptors and aquaporin−2 of the kidney inner medulla resulting in prolonged impairment of the renal capacity to concentrate urine
Free Keywords:kidney, lipopolysaccharide, V2 vasopressin receptors, aquaporin−2, urine concentration, interleukin−1beta, interleukin 6
Last Change of the Resource (YYYY-MM-DD):--
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie
MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie/Gruppe Pavel Osten
MPI für medizinische Forschung/Abteilung Molekulare Neurobiologie/Gruppe Valery Grinevich
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