| ID:
567260.0,
MPI für medizinische Forschung / Abteilung Biomedizinische Optik |
| The actin−myosin interface |
| Translation of Title: | The actin−myosin interface |
| Authors: | Lorenz, Michael; Holmes, Kenneth C. |
| Language: | English |
| Date of Publication (YYYY-MM-DD): | 2010-07-13 |
| Title of Journal: | Proceedings of the National Academy of Sciences of the USA (JSTOR) |
| Journal Abbrev.: | Proc. Natl. Acad. Sci. U. S. A. |
| Volume: | 107 |
| Issue / Number: | 28 |
| Start Page: | 12529 |
| End Page: | 12534 |
| Review Status: | Peer-review |
| Audience: | Experts Only |
| Intended Educational Use: | No |
| Abstract / Description: | In order to understand the mechanism of muscle contraction at the
atomic level, it is necessary to understand how myosin binds to
actin in a reversible way.We have used a novel molecular dynamics
technique constrained by an EM map of the actin−myosin complex
at 13−Å resolution to obtain an atomic model of the strong−binding
(rigor) actin−myosin interface. The constraining force resulting from
the EM map during the molecular dynamics simulation was sufficient
to convert the myosin head from the initial weak−binding
state to the strong−binding (rigor) state. Our actin−myosin model
suggests extensive contacts between actin and the myosin head
(S1). S1 binds to two actin monomers. The contact surface between
actin and S1 has increased dramatically compared with previous
models. A number of loops in S1 and actin are involved in establishing
the interface. Our model also suggests how the loop carrying
the critical Arg 405 Glu mutation in S1 found in a familial cardiomyopathy
might be functionally involved |
| Free Keywords: | cryoelectron microscopy ; myosin II ; myosin V |
| Last Change of the Resource (YYYY-MM-DD): | -- |
| External Publication Status: | published |
| Document Type: | Article |
| Version Comment: | Automatic journal name synchronization |
| Communicated by: | Wulf Kaiser |
| Affiliations: | MPI für medizinische Forschung/Abteilung Biophysik/Protein - Kristallographie XDS
MPI für medizinische Forschung/Abteilung Biophysik/Muskelforschung
MPI für medizinische Forschung/Abteilung Biophysik
MPI für medizinische Forschung/Abteilung Biomedizinische Optik
|
| Identifiers: | LOCALID:7617
URI:http%3A%2F%2Fwww.pnas.org%2Fcontent%2F107%2F28%2F1...
URI:http%3A%2F%2Fwww.pnas.org%2Fcontent%2F107%2F28%2F1...
URI:http%3A%2F%2Fwww.pnas.org%2Fcontent%2F107%2F28%2F1...
DOI:10.1073%2Fpnas.1003604107 |
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