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          Institute: MPI für medizinische Forschung     Collection: Abteilung Biomedizinische Optik     Display Documents

ID: 567260.0, MPI für medizinische Forschung / Abteilung Biomedizinische Optik
The actin−myosin interface
Translation of Title:The actin−myosin interface
Authors:Lorenz, Michael; Holmes, Kenneth C.
Date of Publication (YYYY-MM-DD):2010-07-13
Title of Journal:Proceedings of the National Academy of Sciences of the USA (JSTOR)
Journal Abbrev.:Proc. Natl. Acad. Sci. U. S. A.
Issue / Number:28
Start Page:12529
End Page:12534
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:In order to understand the mechanism of muscle contraction at the
atomic level, it is necessary to understand how myosin binds to
actin in a reversible way.We have used a novel molecular dynamics
technique constrained by an EM map of the actin−myosin complex
at 13−Å resolution to obtain an atomic model of the strong−binding
(rigor) actin−myosin interface. The constraining force resulting from
the EM map during the molecular dynamics simulation was sufficient
to convert the myosin head from the initial weak−binding
state to the strong−binding (rigor) state. Our actin−myosin model
suggests extensive contacts between actin and the myosin head
(S1). S1 binds to two actin monomers. The contact surface between
actin and S1 has increased dramatically compared with previous
models. A number of loops in S1 and actin are involved in establishing
the interface. Our model also suggests how the loop carrying
the critical Arg 405 Glu mutation in S1 found in a familial cardiomyopathy
might be functionally involved
Free Keywords:cryoelectron microscopy ; myosin II ; myosin V
Last Change of the Resource (YYYY-MM-DD):--
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biophysik/Protein - Kristallographie XDS
MPI für medizinische Forschung/Abteilung Biophysik/Muskelforschung
MPI für medizinische Forschung/Abteilung Biophysik
MPI für medizinische Forschung/Abteilung Biomedizinische Optik
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