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          Institute: MPI für medizinische Forschung     Collection: Abteilung Biomedizinische Optik     Display Documents

ID: 567343.0, MPI für medizinische Forschung / Abteilung Biomedizinische Optik
A novel type of interplexiform amacrine cell in the mouse retina
Translation of Title:A novel type of interplexiform amacrine cell in the mouse retina
Authors:Dedek, K.; Breuninger, Tobias; de Sevilla Müller, L. P.; Maxeiner, S.; Schultz, K.; Janssen−Bienhold, U.; Willecke, K.; Euler, Thomas; Weiler, R.
Date of Publication (YYYY-MM-DD):2009-07-01
Title of Journal:European Journal of Neuroscience
Journal Abbrev.:Eur. J. Neurosci.
Issue / Number:2
Start Page:217
End Page:228
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Mammalian retinas comprise an enormous variety of amacrine cells with distinct properties and functions. The present paper
describes a new interplexiform amacrine cell type in the mouse retina. A transgenic mouse mutant was used that expressed the gene
for the enhanced green fluorescent protein (EGFP) instead of the coding DNA of connexin45 in several retinal cell classes, among
which a single amacrine cell population was most prominently labelled. Staining for EGFP and different marker proteins showed that
these amacrine cells are interplexiform: they stratify in stratum S4 ⁄ 5 of the inner plexiform layer and send processes to the outer
plexiform layer. These cells were termed IPA−S4 ⁄ 5 cells. They belong to the group of medium−field amacrine cells and are coupled
homologously and heterologously to other amacrine cells by connexin45. Immunostaining revealed that IPA−S4 ⁄ 5 cells are
GABAergic and express GAT−1, a plasma−membrane−bound GABA transporter possibly involved in non−vesicular GABA release. To
characterize the light responses of IPA−S4 ⁄ 5 cells, patch−clamp recordings in retinal slices were made. Consistent with their
stratification in the ON sublamina of the inner plexiform layer, cells depolarized in response to light ON stimuli and transiently
hyperpolarized in response to light OFF. Responses of cells to green (578 nm) and blue (400 nm) light suggest that they receive
input from cone bipolar cells contacting both M− and S−cones, possibly with reduced S−cone input. A new type of interplexiform ON
amacrine cell is described, which is strongly coupled and uses GABA but not dopamine as its neurotransmitter
Free Keywords:amacrine cell, connexin45, dye coupling, GABAergic, light response
Last Change of the Resource (YYYY-MM-DD):--
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biomedizinische Optik
MPI für medizinische Forschung/Abteilung Biomedizinische Optik/Signalverarbeitung in der Retina
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