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Home Search Quick Search Advanced Fulltext Browse Collections Persons My eDoc Session History Login Name: Password: Documentation Help Support Wiki Direct access to document ID:	Institute: MPI für medizinische Forschung     Collection: Heme and Flavin Enzymes     Display Documents ID: 568098.0, MPI für medizinische Forschung / Heme and Flavin Enzymes Femtosecond X−ray protein nanocrystallography Translation of Title: Femtosecond X−ray protein nanocrystallography Authors: Chapman, Henry N.; Fromme, Petra; Barty, Anton; White, Thomas A.; Kirian, Richard A.; Aquila, Andrew; Schorb, Sebastian; Fromme, Raimund; Marchesini, Stefano; Hunter, Mark S.; Rupp, Daniela; Adolph, Marcus; Gorkhover, Tais; Andersson, Inger; Hirsemann, Helmut; Potdevin, Guillaume; Graafsma, Heinz; Nilsson, Björn; Spence, John C. H.; Schulz, Joachim; DePonte, Daniel P.; Weierstall, Uwe; Doak, R. Bruce; Maia, Filipe R. N. C.; Martin, Andrew V.; Schlichting, Ilme; Lomb, Lukas; Coppola, Nicola; Shoeman, Robert L.; Epp, Sascha W.; Hartmann, Robert; Rolles, Daniel; Rudenko, Artem; Foucar, Lutz; Kimmel, Nils; Weidenspointner, Georg; Holl, Peter; Liang, Mengning; Barthelmess, Miriam; Caleman, Carl; Boutet, Sébastien; Bogan, Michael J.; Krzywinski, Jacek; Bostedt, Christoph; Bajt, Saša; Gumprecht, Lars; Rudek, Benedikt; Erk, Benjamin; Schmidt, Carlo; Hömke, André; Reich, Christian; Pietschner, Daniel; Strüder, Lothar; Hauser, Günter; Gorke, Hubert; Ullrich, Joachim; Herrmann, Sven; Schaller, Gerhard; Schopper, Florian; Soltau, Heike; Kühnel, Kai−Uwe; Messerschmidt, Marc; Bozek, John D.; Hau−Riege, Stefan P.; Frank, Matthias; Hampton, Christina Y.; Sierra, Raymond G.; Starodub, Dmitri; Williams, Garth J.; Hajdu, Janos; Timneanu, Nicusor; Seibert, M. Marvin; Andreasson, Jakob; Rocker, Andrea; Jönsson, Olof; Svenda, Martin; Stern, Stephan; Nass, Karol; Andritschke, Robert; Schröter, Klaus−Dieter; Krasniqi, Faton; Bott, Mario; Schmidt, Kevin E.; Wang, Xiaoyu; Grotjohann, Ingo; Holton, James M.; Barends, Thomas; Neutze, Richard Language: English Date of Publication (YYYY-MM-DD): 2011-02-03 Title of Journal: Nature Journal Abbrev.: Nat. Volume: 470 Issue / Number: 7332 Start Page: 73 End Page: 77 Review Status: Peer-review Audience: Experts Only Intended Educational Use: No Abstract / Description: X−ray crystallography provides the vast majority of macromolecular structures, but the success of the method relies on growing crystals of sufficient size. In conventional measurements, the necessary increase in X−ray dose to record data from crystals that are too small leads to extensive damage before a diffraction signal can be recorded. It is particularly challenging to obtain large, well−diffracting crystals of membrane proteins, for which fewer than 300 unique structures have been determined despite their importance in all living cells. Here we present a method for structure determination where single−crystal X−ray diffraction ‘snapshots' are collected from a fully hydrated stream of nanocrystals using femtosecond pulses from a hard−X−ray free−electron laser, the Linac Coherent Light Source. We prove this concept with nanocrystals of photosystem I, one of the largest membrane protein complexes. More than 3,000,000 diffraction patterns were collected in this study, and a three−dimensional data set was assembled from individual photosystem I nanocrystals (˜200 nm to 2 μm in size). We mitigate the problem of radiation damage in crystallography by using pulses briefer than the timescale of most damage processes. This offers a new approach to structure determination of macromolecules that do not yield crystals of sufficient size for studies using conventional radiation sources or are particularly sensitive to radiation damage Last Change of the Resource (YYYY-MM-DD): -- External Publication Status: published Document Type: Article Communicated by: Wulf Kaiser Affiliations: MPI für medizinische Forschung/Abteilung Biophysik MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Molecular chaperones MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Heme and Flavin Enzymes MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/mRNA Processing MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Analytical Protein Biochemistry MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Coherent diffractive imaging Identifiers: LOCALID:7649 URI:http%3A%2F%2Fwww.nature.com%2Fnature%2Fjournal%2Fv... URI:http%3A%2F%2Fwww.nature.com%2Fnature%2Fjournal%2Fv... URI:http%3A%2F%2Fwww.nature.com%2Fnature%2Fjournal%2Fv... DOI:10.1038%2Fnature09750 Full Text: Sorry, no privileges The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.