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          Institute: MPI für medizinische Forschung     Collection: Analytical Protein Biochemistry     Display Documents

ID: 568198.0, MPI für medizinische Forschung / Analytical Protein Biochemistry
Comparison of recombinant human immunodeficiency virus gag precursor and gag/env fusion proteins and a synthetic env peptide as diagnostic reagents
Translation of Title:Comparison of recombinant human immunodeficiency virus gag precursor and gag/env fusion proteins and a synthetic env peptide as diagnostic reagents
Authors:Shoeman, Robert L.; Young, D.; Pottathil, R.; Victor, J.; Conroy, R. R.; Crowl, R. M.; Coleman, Timothy; Heimer, E.; Lai, C. Y.; Ganguly, K.; Reddy, E. P.; Salka, A. M.; Pine, P. R.; Khan, F. R.; Weissbach, Herbert
Date of Publication (YYYY-MM-DD):1987-03-01
Title of Journal:Analytical Biochemistry
Journal Abbrev.:Anal. Biochem.
Issue / Number:2
Start Page:370
End Page:379
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Diagnostic reagents for detection of human immunodeficiency virus (HIV) exposure with improved reliability may be provided by viral encoded proteins produced by recombinant DNA techniques or by synthetic peptides corresponding to appropriate viral epitopes. We have expressed at high levels in E. coli a gag gene segment corresponding to not, vert, similar97% of the p55 gag precursor protein, as well as a novel gag/env fusion protein that contains antigenic determinants in common with gag p24, env gp41, and env gp120. The gag and gag/env proteins were purified from insoluble inclusion bodies by sequential extraction with increasing concentrations of urea. These components were tested for reactivity with antisera to HIV proteins and peptides. We have also chemically synthesized a peptide corresponding to env residues 578−608, representing a portion of env gp41. The final preparation of gag and gag/env proteins in 8 Image urea reacted with sheep anti−HTLV−III p24 gag antibodies and acquired immune deficiency syndrome (AIDS) patient sera. The gag/env fusion protein also reacted with rabbit anti−HIV env 500−511 peptide antibody. Both recombinant proteins and the env peptide were suitable as reagents for evaluation of serum samples by enzyme−linked immunosorbent assay (ELISA). Results of ELISA assays utilizing the recombinant viral proteins and synthetic peptide were in good agreement with results obtained using disrupted virus as antigen in ELISA assays and immunoblotting
Free Keywords:human immunodeficiency virus; HIV gag protein; HIV gag/env protein; HIV env peptide; inclusion bodies; enzyme−linked immunosorbent assay
Last Change of the Resource (YYYY-MM-DD):--
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Analytical Protein Biochemistry
MPI für medizinische Forschung/Abteilung Biomolekulare Mechanismen/Coherent diffractive imaging
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