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          Institute: MPI für medizinische Forschung     Collection: Abteilung Molekulare Zellforschung     Display Documents



ID: 568825.0, MPI für medizinische Forschung / Abteilung Molekulare Zellforschung
A novel Ca2+−dependent step in exocytosis subsequent to vesicle fusion
Translation of Title:A novel Ca2+−dependent step in exocytosis subsequent to vesicle fusion
Authors:Hartmann, Jana; Lindau, Manfred
Language:English
Date of Publication (YYYY-MM-DD):1995-01-01
Title of Journal:FEBS Letters
Journal Abbrev.:FEBS Lett.
Volume:363
Issue / Number:3
Start Page:217
End Page:220
Review Status:Peer-review
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Exocytosis begins with formation of a small fusion pore which then expands allowing rapid release of granular contents. We studied the influence of cytoplasmic free Ca2+ ([Ca2+]i) on the conductance of the initial pore and on the dynamics of subsequent expansion in horse eosinophils using the patch clamp technique. The mean initial conductance is not, vert, similar200 pS independent of [Ca2+]i. This value is close to that previously found in beige mouse mast cells. The pore subsequently expands by 18 nS/s at [Ca2+]i < 10 nM, by 40 nS/s at [Ca2+]i = 1.5 μM and by 90 nS/s at [Ca2+]i = 10 μM. These results show that the structure of the initial fusion pore is independent of cytoplasmic Ca2+. However, the pore expansion is a Ca2+−dependent process modulating secretion at a step later than vesicle−plasma membrane fusion
Free Keywords:Exocytosis; Calcium; Eosinophil; Fusion pore
Last Change of the Resource (YYYY-MM-DD):--
External Publication Status:published
Document Type:Article
Communicated by:Wulf Kaiser
Affiliations:MPI für medizinische Forschung/Abteilung Molekulare Zellforschung
Identifiers:LOCALID:6619
URI:http%3A%2F%2Fwww.sciencedirect.com%2Fscience%3F_ob...
URI:http%3A%2F%2Fwww.sciencedirect.com%2Fscience%3F_ob...
DOI:doi%3A10.1016%2F0014-5793%2895%2900318-4%20
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