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          Institute: MPI für Dynamik komplexer technischer Systeme     Collection: Bioprocess Engineering     Display Documents

ID: 571773.0, MPI für Dynamik komplexer technischer Systeme / Bioprocess Engineering
Tools for the Glycome Analysis in the Post-Genomic Era
Authors:Rapp, E.; Hennig, R.; Borowiak, M.; Kottler, R.; Reichl, U.
Name of Conference/Meeting:7th ISABS Conference in Forensic, Anthropologic and Medical Genetics and Mayo Clinic Lectures in Translational Medicine
Place of Conference/Meeting:Bol, Island of Brač, Croatia
(Start) Date of Conference/Meeting
End Date of Conference/Meeting 
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Glycosylation is a post-translational modification that enriches protein complexity and function. Dysregulation of glycosylation is associated with a wide range of diseases, including cancer, diabetes, as well as congenital, cardiovascular, immunological and infectious disorders. With regard to biotechnology, proper glycosylation of biologicals is important, as deviations in glycosylation are known to be associated with adverse drug reactions and reduced therapeutic efficacy. Therefore, glycomics is a rapidly emerging field that can be viewed as a complement to other „omics“ approaches including proteomics and genomics. There is a dramatic dynamic increase in the demand for sophisticated databases and analytical tools in glycobiology respectively glycobiotechnology. However, glycomics is significantly lagging behind genomics and proteomics, mainly due to the absence of high-throughput analytical methods which can reliably quantify a multitude of glycan structures in complex biological samples. For that reason, the EU-wide collaborative project “Methods for high-throughput (HTP) analysis of protein glycosylation” is funded within the FP7 of the European Union and will be started in 2011. The project is designed to fill that technology gap through development of relevant, reliable, and affordable commercial HTP glycoanalysis tools. This modular glycoprofiling system – to be developed and established within the project – is based on three orthogonal analysis platforms (UPLC, MS and CGE-LIF). Project and analysis platforms will be presented, focussing on the glycoanalysis approach, based on multiplexed capillary gel electrophoresis with laser induced fluorescence detection (CGE-LIF) utilizing a DNA-sequencer, which shows high potential for high-throughput (HTP) glycoprofiling of glycoconjugates.
Document Type:Talk at Event
Communicated by:Udo Reichl
Affiliations:MPI für Dynamik komplexer technischer Systeme/Bioprocess Engineering
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