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          Institute: MPI für Infektionsbiologie     Collection: Department of Molecular Biology     Display Documents



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ID: 574264.0, MPI für Infektionsbiologie / Department of Molecular Biology
Phylogeography and Molecular Epidemiology of Yersinia pestis in Madagascar
Authors:Vogler, Amy J.; Chan, Fabien; Wagner, David M.; Roumagnac, Philippe; Lee, Judy; Nera, Roxanne; Eppinger, Mark; Ravel, Jacques; Rahalison, Lila; Rasoamanana, Bruno W.; Beckstrom-Sternberg, Stephen M.; Achtman, Mark; Chanteau, Suzanne; Keim, Paul
Language:English
Date of Publication (YYYY-MM-DD):2011-09
Title of Journal:PLoS Neglected Tropical Diseases
Journal Abbrev.:Plos Neglect. Trop. Dis.
Volume:5
Issue / Number:9
Sequence Number of Article:e1319
Copyright:Copyright: © 2011 Vogler et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:Background: Plague was introduced to Madagascar in 1898 and continues to be a significant human health problem. It exists mainly in the central highlands, but in the 1990s was reintroduced to the port city of Mahajanga, where it caused extensive human outbreaks. Despite its prevalence, the phylogeography and molecular epidemiology of Y. pestis in Madagascar has been difficult to study due to the great genetic similarity among isolates. We examine island-wide geographic-genetic patterns based upon whole-genome discovery of SNPs, SNP genotyping and hypervariable variable-number tandem repeat (VNTR) loci to gain insight into the maintenance and spread of Y. pestis in Madagascar. Methodology/Principal Findings: We analyzed a set of 262 Malagasy isolates using a set of 56 SNPs and a 43-locus multi-locus VNTR analysis (MLVA) system. We then analyzed the geographic distribution of the subclades and identified patterns related to the maintenance and spread of plague in Madagascar. We find relatively high levels of VNTR diversity in addition to several SNP differences. We identify two major groups, Groups I and II, which are subsequently divided into 11 and 4 subclades, respectively. Y. pestis appears to be maintained in several geographically separate subpopulations. There is also evidence for multiple long distance transfers of Y. pestis, likely human mediated. Such transfers have resulted in the reintroduction and establishment of plague in the port city of Mahajanga, where there is evidence for multiple transfers both from and to the central highlands. Conclusions/Significance: The maintenance and spread of Y. pestis in Madagascar is a dynamic and highly active process that relies on the natural cycle between the primary host, the black rat, and its flea vectors as well as human activity.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:Beate Löhr
Affiliations:MPI für Infektionsbiologie/Department of Molecular Biology
External Affiliations:[Vogler, AJ; Wagner, DM; Lee, J; Nera, R; Beckstrom-Sternberg, SM; Keim, P] No Arizona Univ, Ctr Microbial Genet & Genom, Flagstaff, AZ 86011 USA.; [Chan, F; Rahalison, L; Rasoamanana, BW; Chanteau, S] Inst Pasteur Madagascar, Antananarivo, Madagascar.; [Eppinger, M; Ravel, J] Univ Maryland, Sch Med, Inst Genom Sci IGS, Baltimore, MD 21201 USA.; [Beckstrom-Sternberg, SM; Keim, P] Translat Genom Res Inst, Phoenix, AZ USA.; [Achtman, M] Univ Coll Cork, Environm Res Inst, Cork, Ireland.
Identifiers:ISI:000296578900027 [ID No:1]
ISSN:1935-2727 [ID No:2]
DOI:10.1371/journal.pntd.0001319 [ID No:3]
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