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          Institute: MPI für Biophysik     Collection: Abt. Biophysikalische Chemie     Display Documents



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ID: 580511.0, MPI für Biophysik / Abt. Biophysikalische Chemie
Optogenetic Long-Term Manipulation of Behavior and Animal Development
Authors:Schultheis, Christian; Liewald, Jana Fiona; Bamberg, Ernst; Nagel, Georg; Gottschalk, Alexander
Language:English
Date of Publication (YYYY-MM-DD):2011-04-20
Title of Journal:PLoS ONE
Journal Abbrev.:Plos One
Volume:6
Issue / Number:4
Start Page:1
End Page:9
Sequence Number of Article:e18766. doi:10.1371/journal.pone.0018766
Review Status:Peer-review
Audience:Experts Only
Abstract / Description:Channelrhodopsin-2 (ChR2) is widely used for rapid photodepolarization of neurons, yet, as it requires high-intensity blue light for activation, it is not suited for long-term in vivo applications, e.g. for manipulations of behavior, or photoactivation of neurons during development. We used “slow” ChR2 variants with mutations in the C128 residue, that exhibit delayed off-kinetics and increased light sensitivity in Caenorhabditis elegans. Following a 1 s light pulse, we could photodepolarize neurons and muscles for minutes (and with repeated brief stimulation, up to days) with low-intensity light. Photoactivation of ChR2(C128S) in command interneurons elicited long-lasting alterations in locomotion. Finally, we could optically induce profound changes in animal development: Long-term photoactivation of ASJ neurons, which regulate larval growth, bypassed the constitutive entry into the “dauer” larval state in daf-11 mutants. These lack a guanylyl cyclase, which possibly renders ASJ neurons hyperpolarized. Furthermore, photostimulated ASJ neurons could acutely trigger dauer-exit. Thus, slow ChR2s can be employed to long-term photoactivate behavior and to trigger alternative animal development.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:N. N.
Affiliations:MPI für Biophysik/Abteilung Biophysikalische Chemie
External Affiliations:Institute of Biochemistry, Goethe University Frankfurt, Frankfurt am Main, Germany;
Frankfurt Institute for Molecular Life Sciences (FMLS), Goethe University Frankfurt, Frankfurt am Main, Germany;
Botanik I, University of Würzburg, Würzburg, Germany
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