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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2011-arch     Display Documents



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ID: 585166.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2011-arch
Fluorescence imaging of single Kinesin motors on immobilized microtubules.
Authors:Korten, Till; Nitzsche, Bert; Gell, Christopher; Ruhnow, Felix; Leduc, Cecile; Diez, Stefan
Date of Publication (YYYY-MM-DD):2011
Title of Journal:Methods in Molecular Biology (Clifton, N.J.)
Volume:783
Start Page:121
End Page:137
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Recent developments in optical microscopy and nanometer tracking have greatly improved our understanding of cytoskeletal motor proteins. Using fluorescence microscopy, dynamic interactions are now routinely observed in vitro on the level of single molecules mainly using a geometry, where fluorescently labeled motors move on surface-immobilized filaments. In this chapter, we review recent methods related to single-molecule kinesin motility assays. In particular, we aim to provide practical advice on: how to set up the assays, how to acquire high-precision data from fluorescently labeled kinesin motors and attached quantum dots, and how to analyze data by nanometer tracking.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:thuem
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:4516
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