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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2011-arch     Display Documents



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ID: 585189.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2011-arch
Bottom-up shotgun lipidomics by higher energy collisional dissociation on LTQ Orbitrap mass spectrometers.
Authors:Schuhmann, Kai; Herzog, Ronny; Schwudke, Dominik; Metelmann-Strupat, Wolfgang; Bornstein, Stefan R.; Shevchenko, Andrej
Date of Publication (YYYY-MM-DD):2011
Title of Journal:Analytical Chemistry
Volume:83
Issue / Number:14
Start Page:5480
End Page:5487
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Higher energy collision dissociation (HCD) is a complementary fragmentation tool that has recently become available on mass spectrometers of the LTQ Orbitrap family. We report on a shotgun bottom-up lipidomics approach that relies on HCD of the isolated lipid precursors. HCD, together with the high mass resolution and mass accuracy of the Orbitrap analyzer, improved the confidence of molecular species assignment and accuracy of their quantification in total lipid extracts. These capabilities were particularly important for accounting for biologically interesting lipid species comprising polyunsaturated and odd numbered fatty acid moieties. We argue that now both bottom-up and top-down shotgun lipidomics could be performed on the same instrumentation platform.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:thuem
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:4688
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