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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2011-arch     Display Documents



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ID: 585193.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2011-arch
Under-filling trapping objectives optimizes the use of the available laser power in optical tweezers.
Authors:Mahamdeh, Mohammed; Perez Campos, Citlali; Schäffer, Erik
Date of Publication (YYYY-MM-DD):2011
Title of Journal:Optics Express
Volume:19
Issue / Number:12
Start Page:11759
End Page:11768
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:For optical tweezers, especially when used in biological studies, optimizing the trapping efficiency reduces photo damage or enables the generation of larger trapping forces. One important, yet not-well understood, tuning parameter is how much the laser beam needs to be expanded before coupling it into the trapping objective. Here, we measured the trap stiffness for 0.5-2 μm-diameter microspheres for various beam expansions. We show that the highest overall trapping efficiency is achieved by slightly under-filling a high-numerical aperture objective when using microspheres with a diameter corresponding to about the trapping-laser wavelength in the medium. The optimal filling ratio for the lateral direction depended on the microsphere size, whereas for the axial direction it was nearly independent. Our findings are in agreement with Mie theory calculations and suggest that apart from the choice of the optimal microsphere size, slightly under-filling the objective is key for the optimal performance of an optical trap.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:thuem
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:4668
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