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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2011-arch     Display Documents



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ID: 585195.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2011-arch
Tracking single particles and elongated filaments with nanometer precision.
Authors:Ruhnow, Felix; Zwicker, David; Diez, Stefan
Date of Publication (YYYY-MM-DD):2011
Title of Journal:Biophysical Journal
Volume:100
Issue / Number:11
Start Page:2820
End Page:2828
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Recent developments in image processing have greatly advanced our understanding of biomolecular processes in vitro and in vivo. In particular, using Gaussian models to fit the intensity profiles of nanometer-sized objects have enabled their two-dimensional localization with a precision in the one-nanometer range. Here, we present an algorithm to precisely localize curved filaments whose structures are characterized by subresolution diameters and micrometer lengths. Using surface-immobilized microtubules, fluorescently labeled with rhodamine, we demonstrate positional precisions of ∼2 nm when determining the filament centerline and ∼9 nm when localizing the filament tips. Combined with state-of-the-art single particle tracking we apply the algorithm 1), to motor-proteins stepping on immobilized microtubules, 2), to depolymerizing microtubules, and 3), to microtubules gliding over motor-coated surfaces.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:thuem
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:4494
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