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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2011-arch     Display Documents



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ID: 585197.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2011-arch
Restoration of Uneven Illumination in Light Sheet Microscopy Images.
Authors:Uddin, Mohammad Shorif; Lee, Hwee Kuan; Preibisch, Stephan W.; Tomancak, Pavel
Date of Publication (YYYY-MM-DD):2011
Title of Journal:Microscopy and Microanalysis
Volume:17
Issue / Number:4
Start Page:607
End Page:613
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:Light microscopy images suffer from poor contrast due to light absorption and scattering by the media. The resulting decay in contrast varies exponentially across the image along the incident light path. Classical space invariant deconvolution approaches, while very effective in deblurring, are not designed for the restoration of uneven illumination in microscopy images. In this article, we present a modified radiative transfer theory approach to solve the contrast degradation problem of light sheet microscopy (LSM) images. We confirmed the effectiveness of our approach through simulation as well as real LSM images.
External Publication Status:published
Document Type:Article
Version Comment:Automatic journal name synchronization
Communicated by:thuem
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:4493
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