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Home Search Quick Search Advanced Fulltext Browse Collections Persons My eDoc Session History Login Name: Password: Documentation Help Support Wiki Direct access to document ID:	Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2011-arch     Display Documents ID: 585213.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2011-arch A high-resolution C. elegans essential gene network based on phenotypic profiling of a complex tissue. Authors: Green, Rebecca A; Kao, Huey-Ling; Audhya, Anjon; Arur, Swathi; Mayers, Jonathan R; Fridolfsson, Heidi N; Schulman, Monty; Schloissnig, Siegfried; Niessen, Sherry; Laband, Kimberley; Wang, Shaohe; Starr, Daniel A; Hyman, Anthony A.; Schedl, Tim; Desai, Arshad; Piano, Fabio; Gunsalus, Kristin C.; Oegema, Karen Date of Publication (YYYY-MM-DD): 2011 Title of Journal: Cell Volume: 145 Issue / Number: 3 Start Page: 470 End Page: 482 Copyright: not available Audience: Experts Only Intended Educational Use: No Abstract / Description: High-content screening for gene profiling has generally been limited to single cells. Here, we explore an alternative approach-profiling gene function by analyzing effects of gene knockdowns on the architecture of a complex tissue in a multicellular organism. We profile 554 essential C. elegans genes by imaging gonad architecture and scoring 94 phenotypic features. To generate a reference for evaluating methods for network construction, genes were manually partitioned into 102 phenotypic classes, predicting functions for uncharacterized genes across diverse cellular processes. Using this classification as a benchmark, we developed a robust computational method for constructing gene networks from high-content profiles based on a network context-dependent measure that ranks the significance of links between genes. Our analysis reveals that multi-parametric profiling in a complex tissue yields functional maps with a resolution similar to genetic interaction-based profiling in unicellular eukaryotes-pinpointing subunits of macromolecular complexes and components functioning in common cellular processes. External Publication Status: published Document Type: Article Communicated by: thuem Affiliations: MPI für molekulare Zellbiologie und Genetik Identifiers: LOCALID:4618 The scope and number of records on eDoc is subject to the collection policies defined by each institute - see "info" button in the collection browse view.