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          Institute: MPI für molekulare Zellbiologie und Genetik     Collection: Publikationen MPI-CBG 2011-arch     Display Documents



ID: 585228.0, MPI für molekulare Zellbiologie und Genetik / Publikationen MPI-CBG 2011-arch
Cortical constriction during abscission involves helices of ESCRT-III-dependent filaments.
Authors:Guizetti, Julien; Schermelleh, Lothar; Mäntler, Jana; Maar, Sandra; Poser, Ina; Leonhardt, Heinrich; Müller-Reichert, Thomas; Gerlich, Daniel W
Date of Publication (YYYY-MM-DD):2011
Title of Journal:Science (New York, N.Y.)
Volume:331
Issue / Number:6024
Start Page:1616
End Page:1620
Copyright:not available
Audience:Experts Only
Intended Educational Use:No
Abstract / Description:After partitioning of cytoplasmic contents by cleavage furrow ingression, animal cells remain connected by an intercellular bridge, which subsequently splits by abscission. Here, we examined intermediate stages of abscission in human cells by using live imaging, three-dimensional structured illumination microscopy, and electron tomography. We identified helices of 17-nanometer-diameter filaments, which narrowed the cortex of the intercellular bridge to a single stalk. The endosomal sorting complex required for transport (ESCRT)-III co-localized with constriction zones and was required for assembly of 17-nanometer-diameter filaments. Simultaneous spastin-mediated removal of underlying microtubules enabled full constriction at the abscission site. The identification of contractile filament helices at the intercellular bridge has broad implications for the understanding of cell division and of ESCRT-III-mediated fission of large membrane structures.
External Publication Status:published
Document Type:Article
Communicated by:thuem
Affiliations:MPI für molekulare Zellbiologie und Genetik
Identifiers:LOCALID:4671
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