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          Institute: MPI für Entwicklungsbiologie     Collection: Abteilung 1 - Protein Evolution (A. Lupas)     Display Documents



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ID: 591402.0, MPI für Entwicklungsbiologie / Abteilung 1 - Protein Evolution (A. Lupas)
Improving the resistance of a eukaryotic beta-barrel protein to thermal and chemical perturbations
Authors:Gessmann, D.; Mager, F.; Naveed, H.; Arnold, T.; Weirich, S.; Linke, D.; Liang, J.; Nussberger, S.
Date of Publication (YYYY-MM-DD):2011-10-14
Title of Journal:J Mol Biol
Volume:413
Issue / Number:1
Start Page:150
End Page:161
Review Status:not specified
Audience:Not Specified
Abstract / Description:beta-Barrel membrane proteins have regular structures with extensive hydrogen-bond networks between their transmembrane (TM) beta-strands, which stabilize their protein fold. Nevertheless, weakly stable TM regions, which are important for the protein function and interaction with other proteins, exist. Here, we report on the apparent stability of human Tom40A, a member of the "mitochondrial porin family" and main constituent of the mitochondrial protein-conducting channel TOM (translocase of the outer membrane). Using a physical interaction model, TmSIP, for beta-barrel membrane proteins, we have identified three unfavorable beta-strands in the TM domain of the protein. Substitution of key residues inside these strands with hydrophobic amino acids results in a decreased sensitivity of the protein to chemical and/or thermal denaturation. The apparent melting temperature observed when denatured at a rate of 1 degrees C per minute is shifted from 73 to 84 degrees C. Moreover, the sensitivity of the protein to denaturant agents is significantly lowered. Further, we find a reduced tendency for the mutated protein to form dimers. We propose that the identified weakly stable beta-strands 1, 2 and 9 of human Tom40A play an important role in quaternary protein-protein interactions within the mammalian TOM machinery. Our results show that the use of empirical energy functions to model the apparent stability of beta-barrel membrane proteins may be a useful tool in the field of nanopore bioengineering.
Free Keywords:Amino Acid Substitution/genetics; Circular Dichroism; Humans; Membrane Transport Proteins/*chemistry/genetics; Mitochondrial Proteins/*chemistry/genetics; Models, Molecular; Mutant Proteins/chemistry/genetics; Protein Denaturation/drug effects/radiation effects; Protein Multimerization; Protein Structure, Secondary; Transition Temperature
External Publication Status:published
Document Type:Article
Affiliations:MPI für Entwicklungsbiologie/Abteilung 1 - Proteinevolution (Andrei Lupas)
External Affiliations:%G eng
Identifiers:ISSN:1089-8638 (Electronic) 0022-2836 (Linking) %R S002... [ID No:1]
URL:http://www.ncbi.nlm.nih.gov/pubmed/21835183 [ID No:2]
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